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A synergistic effect of glutathione-depletion and elicitation on the production of 6-methoxymellein in carrot cells

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Abstract

The effects of buthionine sulfoximine (BSO) and yeast glucan elicitor (YE) on the production of 6-methoxymellein (6-MM) and generation of H2O2 in suspension-cultured carrot cells were examined. Administration of BSO and YE together affected the cells synergistically to lead to an enhanced production of 6-MM. These data indicate the significance of formation and decay of active oxygen species as a second signal of elicitation in triggering the biosynthesis of the phytoalexin.

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Abbreviations

BSO:

buthionine sulfoximine

MDA:

malondialdehyde

6-MM:

6-methoxymellein

YE:

yeast glucan elicitor

References

  • Apostol I, Heinstein PF, Low PS (1989) Plant Physiol. 90: 109–116

    Google Scholar 

  • Atkinson MM, Huang JS, Knopp JA (1985) Phytopathology 75: 1270–1274

    Google Scholar 

  • Barz W, Beimen A, Dräger B, Jaques U, Otto CH, Süper E, Upmeier B (1990) In: Charlwood B V, Rhodes MJC (eds) Secondary products from plant tissue culture, Clarendon Press, Oxford, pp 79–102

    Google Scholar 

  • Creelman RA, Tierney ML, Mullet JE (1992) Proc. Natl. Acad. Sci. USA 89: 4938–4941

    Google Scholar 

  • Doke N, Ohashi Y (1988) Physiol. Mol. Plant Pathol. 32: 163–175

    Google Scholar 

  • Earnshaw BA, Johnson MA (1985) Biochim. Biophys. Res. Commun. 133: 988–993

    Google Scholar 

  • Epperlein MM, Noronha-Dutra AA, Strange RN (1986) Physiol. Mol. Plant. Pathol. 28: 67–77

    Google Scholar 

  • Farmer EE, Ryan CA (1992) Plant Cell 4: 129–134

    Google Scholar 

  • Felix G, Grosskopf DG, Regenass M, Boller T (1991) Proc. Natl. Acad. Sci. USA 88: 8831–8834

    Google Scholar 

  • Gundlach H, Müller MJ, Kutchan TM, Zenk MH (1992) Proc. Natl. Acad. Sci. USA 89: 2389–2393

    Google Scholar 

  • Guo ZJ, Nakagawana N, Sumitani K, Ohta Y (1993) Plant Physiol. 102: 45–51

    Google Scholar 

  • Hahn M G, Albersheim P (1978) Plant Physiol. 62: 107–111

    Google Scholar 

  • Kurosaki F, Matsui K, Nishi A (1984) Physiol. Plant Pathol. 25: 313–322

    Google Scholar 

  • Kurosaki F, Nishi A (1984) Physiol. Plant Pathol. 24: 169–176

    Google Scholar 

  • Marinelli F, Gregorio SD, Ronchi VN (1991) Plant Sci. 77: 261–266

    Google Scholar 

  • Nakagawara S, Nakamura N, Guo ZJ, Sumitani K, Katoh K, Ohta Y (1993) Plant Cell Physiol. 34: 421–429

    Google Scholar 

  • Ramasarma T (1990) Ind. J. Biochem. Biophys. 27: 269–274

    Google Scholar 

  • Rogers KR, Albert F, Anderson AJ (1988) Plant Physiol. 86: 547–553

    Google Scholar 

  • Schwache R, Hager A (1992) Planta 187: 136–141

    Google Scholar 

  • Vera-Estrella R, Blumwald E, Higgins VJ (1992) Plant Physiol. 99: 1208–1215

    Google Scholar 

  • Yang S F, Hoffman N E (1984) Ann. Rev. Plant Physiol. 35: 155–189

    Google Scholar 

  • Young DH, Köhle H, Kauss H (1982) Plant Physiol. 70: 1449–1454

    Google Scholar 

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Communicated by A. Komamine

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Guo, Zj., Ohta, Y. A synergistic effect of glutathione-depletion and elicitation on the production of 6-methoxymellein in carrot cells. Plant Cell Reports 12, 617–620 (1993). https://doi.org/10.1007/BF00232810

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  • DOI: https://doi.org/10.1007/BF00232810

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