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Gene transfer to maize male reproductive structure by particle bombardment of tassel primordia

Plant Cell Reports volume 12, pages 607–611 (1993)Cite this article

Summary

Maize (Zea mays L.) tassel primordia were used as a target for particle bombardment, to assess the possibility of introducing foreign DNA into male reproductive structures. Transient expression of the β-glucuronidase gene (GUS) or anthocyanin marker genes (C1 and B-Peru) driven by the CaMV 35S promoter was obtained in tassel primordia 24h after bombardment. Gold particles coated with DNA reached stamen primordia tissues, which eventually form the anthers and pollen. Bombarded tassels were also cultured in vitro and GUS activity was detected in the vascular tissue of mature anthers that developed within 4 weeks. This new approach represents a preliminary step toward pollen mediated transformation.

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References

  • Alwen A, Eller N, Kastluer M, Benito-Moreno R, Heberle-Bors E (1990) Physiol Plant 79: 194–196

    Google Scholar 

  • Batty NP, Evan JM (1992) Transgenic Research 1: 107–113

    Google Scholar 

  • Chandler VL, Radicella JP, Robbins TP, Chen J, Turks D (1989) Plant Cell 1: 1175–1183

    Google Scholar 

  • Cheng PC, Greyson RI, Walden DB (1983) Amer J Bot 70: 450–462

    Google Scholar 

  • Christou P (1992) The Plant Journal 2: 275–281

    Google Scholar 

  • Coe EH Jr, Neuffer MG, Hoisington DA (1988) In: Corn and corn improvement. Agronomy monograph no. 18, 3rd ed, pp 81–258

  • Dawe RK, Freeling M (1992) Development 116: 1077–1085

    Google Scholar 

  • Goff SA, Klein TM, Roth BA, Fromm ME, Cone KC, Radicella JP, Chandler VL (1990) EMBO J 9: 2517–2522

    Google Scholar 

  • Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR Jr, Willetts NG. (1990) Plant Cell 2: 603–618

    Google Scholar 

  • Hess D (1987) Int Rev Cytol 107: 367–395

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Reporter 5: 387–405

    Google Scholar 

  • Koziel MG, Beland GL, Bowman C, Carozzi NB, Crenshaw R, Crossland L, Dawson J, Desai N, Hill M, Kadwell S, Launis K, Lewis K, Maddox D, McPherson K, Meghji MR, Merlin E, Rhodes R, Warren GW, Wright M, Evola SV (1993) Bio/Technology 11: 194–200

    Google Scholar 

  • Ludwig SR, Bowen B, Beach L, Wessler SR (1990) Science 247: 449–450

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 473–498

    CAS  Google Scholar 

  • Pareddy DR, Greyson RI (1985) Plant Cell Organ Tissue Culture 5: 119–128

    Google Scholar 

  • Pareddy DR, Greyson RI, Walden DB (1987) Planta 170: 141–143

    Google Scholar 

  • Paz-Arez J, Ghosal D, Wienand U, Peterson PA, Saedler H (1987) EMBO 16: 3553–3558

    Google Scholar 

  • Plegt L, Bino RJ (1989) Mol Gen Genet 216: 321–327

    Google Scholar 

  • Radicella JP, Turks D, Chandler VL (1991) Plant Mol Biol 17: 127–130

    Google Scholar 

  • Roeckel P, Moloney MM, Drevet JR (1992) Int Rev Cytol 140: 425–446

    Google Scholar 

  • Sanford J (1990) Physiol Plant 79: 206–209

    Google Scholar 

  • Stöger E, Benito Moreno M, Ylstra B, Vicente O, Heberle-Bors E (1992) Transgenic Research 1: 71–78

    Google Scholar 

  • Twell D, Klein TM, Fromm ME, McCormick S (1989) Plant Physiol 91: 1270–1274

    Google Scholar 

  • White PR (1943) A handbook of plant tissue culture. The Jacques Cattel Press, Lancaster, PA

    Google Scholar 

  • Yamashita T, Iida A, Morikawa H (1991) Plant Physiol 97: 829–831

    Google Scholar 

Download references

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Affiliations

  1. CIBA-GEIGY Agricultural Biotechnology, P.O. Box 12257, 27709-2257, Research Triangle Park, NC, USA

    Isabelle Dupuis & Gary M. Pace

Authors
  1. Isabelle Dupuis
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  2. Gary M. Pace
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Communicated by J. J. Finer

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Dupuis, I., Pace, G.M. Gene transfer to maize male reproductive structure by particle bombardment of tassel primordia. Plant Cell Reports 12, 607–611 (1993). https://doi.org/10.1007/BF00232808

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  • DOI: https://doi.org/10.1007/BF00232808

Key words

  • Transient expression
  • Particle bombardment
  • Tassel primordia
  • In vitro culture
  • Anthers
  • Zea mays