Skip to main content

In vitro shoot proliferation and propagation of guava (Psidium guajava L.) from germinated seedlings


Guava seeds were germinated on Murashige and Skoog (MS) medium with or without 8.8 μM benzyladenine (BA). BA increased the rate of germination and the number of lateral shoots (3.4 vs 1.2 per seedling). Stem nodes from these lateral shoots were cultured on proliferation media with 4.4 μM BA, and multiple shoots (3.5) were formed within 4 weeks of culture. Increasing the concentration of BA or the addition of naphthaleneacetic acid (NAA) did not affect shoot formation. Shoots produced from explants and lateral shoots from germinated seedlings were rooted in media containing activated charcoal (AC) or 9.8 μM indolebutyric acid (IBA). Shoots rooted with IBA had a higher rooting percentage (100% vs 75%) and a greater number of roots (5.5 vs 3.2) but the shoots were shorter (2.6 vs 3.4 cm) than when rooted in AC, and they required an additional 4 weeks of culture in media with AC to achieve shoot elongation. About 80% of the shoots with roots survived in the glasshouse and produced normal phenotypic plants.

This is a preview of subscription content, access via your institution.



activated charcoal




indole-3-butyric acid


Murashige and Skoog (1962) medium


1-naphthaleneacetic acid




  • Amin M, Jaiswal VS (1987) Plant Cell Tiss Org Cult 9:235–244

    Google Scholar 

  • Amin M, Jaiswal VS (1988) Scientia Hort 36:89–95

    Google Scholar 

  • Bourke D (1976) FA O, CAB Hort Rev No 4:530–553

    Google Scholar 

  • International Board for Plant Genetic Resources (1986) Genetic resources of tropical and sub-tropical fruits and nuts. IBGR, Rome.

    Google Scholar 

  • Itao PJ, Nakasone HY (1968) Proc Trop Reg Amer Soc Hort Sci 12:216–221

    Google Scholar 

  • Mohamed-Yasseen Y, Splittstoesser WE (1990) Plant Growth Regul Soc Amer Quart 18:203–210

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Pillay I, Railton ID (1983) Plant Physiol 71:972–974

    Google Scholar 

  • Seshadri TR, Vasishta K (1965) Phytochemistry 4:317–326

    Google Scholar 

Download references

Author information

Authors and Affiliations


Additional information

Communicated by G. C. Phillips

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mohamed-Yasseen, Y., Barringer, S.A., Schnell, R.J. et al. In vitro shoot proliferation and propagation of guava (Psidium guajava L.) from germinated seedlings. Plant Cell Reports 14, 525–528 (1995).

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: