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An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture

Abstract

A thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6–0.7mm thick) obtained by transverse sectioning of a single shoot tip (6–7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6–7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections. PLB developed into plantlets on solid Vacin and Went medium containing 10% (v/v) coconut water and 0.5 g l−1 activated charcoal. With this procedure, more than 80,000 plantlets could be produced from thin sections obtained from a single shoot tip in a year as compared to nearly 11,000 plantlets produced by the conventional shoot tip method.

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Abbreviations

BA:

6-benzyladenine

CD:

callus development

CW:

coconut water

KC:

Knudson C medium

MS:

Murashige and Skoog medium

NAA:

α-naphthaleneacetic acid

PLB:

protocorm-like body

TS:

thin section

VW:

Vacin and Went medium

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Communicated by A. Komamine

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Lakshmanan, P., Loh, CS. & Goh, CJ. An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture. Plant Cell Reports 14, 510–514 (1995). https://doi.org/10.1007/BF00232785

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  • DOI: https://doi.org/10.1007/BF00232785

Key words

  • Aranda Deborah
  • orchids
  • thin section culture
  • tissue culture