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In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant

Plant Cell Reports volume 14, pages 493–496 (1995)Cite this article

Abstract

A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.

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Abbreviations

2iP:

2-isopentenyladenine

BA:

6-benzyladenine

LSD:

Least Significant Difference

NAA:

1-naphthaleneacetic acid

TDZ:

thidiazuron

WPM:

Woody Plant Medium (Lloyd & McCown 1980) basal medium

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Author information

Affiliations

  1. Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, RG6 2AS, Reading, UK

    A. A. Mao, A. Wetten & P. D. S. Caligari

  2. Royal Botanic Gardens, TW9 3AE, Kew, Richmond, Surrey, UK

    M. Fay

Authors
  1. A. A. Mao
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  2. A. Wetten
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  3. M. Fay
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  4. P. D. S. Caligari
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Additional information

Communicated by M.R. Davey

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Mao, A.A., Wetten, A., Fay, M. et al. In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant. Plant Cell Reports 14, 493–496 (1995). https://doi.org/10.1007/BF00232781

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  • DOI: https://doi.org/10.1007/BF00232781

Keywords

  • Medicinal Plant
  • Chromosome Number
  • Propagation System
  • Clonal Propagation
  • Shoot Apex