Abstract
A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.
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Abbreviations
- 2iP:
-
2-isopentenyladenine
- BA:
-
6-benzyladenine
- LSD:
-
Least Significant Difference
- NAA:
-
1-naphthaleneacetic acid
- TDZ:
-
thidiazuron
- WPM:
-
Woody Plant Medium (Lloyd & McCown 1980) basal medium
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Communicated by M.R. Davey
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Mao, A.A., Wetten, A., Fay, M. et al. In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant. Plant Cell Reports 14, 493–496 (1995). https://doi.org/10.1007/BF00232781
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DOI: https://doi.org/10.1007/BF00232781