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Plant Cell Reports

, Volume 14, Issue 10, pp 616–619 | Cite as

Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants

  • Leandro Peña
  • Magdalena Cervera
  • José Juárez
  • Antonio Navarro
  • José A. Pina
  • Nuria Durán-Vila
  • Luis Navarro
Article

Summary

Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.

Key words

Citrus sinensis L. Osbeck cv. Pineapple Sweet orange Agrobacterium tumefaciens Genetic transformation 

Abbreviations

BA

benzyladenine

CaMV

cauliflowermosaic virus

GUS

ß-glucuronidase

LB

Luria Broth

MS

Murashige and Skoog

NAA

naphthalenacetic acid

NOS

nopaline synthase

NPT II

neomycin phosphotransferase II

PEG

polyethylene glycol

RM

rooting medium

SRM

shoot regeneration medium

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Leandro Peña
    • 1
  • Magdalena Cervera
    • 1
  • José Juárez
    • 1
  • Antonio Navarro
    • 1
  • José A. Pina
    • 1
  • Nuria Durán-Vila
    • 1
  • Luis Navarro
    • 1
  1. 1.Dpto. de protección vegetal y biotecnologíaInstitute Valenciano de Investigaciones AgrariasMoncadaSpain

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