Skip to main content
SpringerLink
Log in

Agrobacterium-mediated transformation of peanut (Arachis hypogaea L.) embryo axes and the development of transgenic plants

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Transgenic peanut (Arachis hypogaea L.) plants have been produced using an Agrobacterium-mediated transformation system. Zygotic embryo axes from mature seed were cocultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector that contained the genes for the scorable marker B-glucuronidase (GUS) and the selectable marker neomycin phosphotransferase II. Nine percent of the germinated seedlings were GUS+. Polymerase chain reaction analysis confirmed that GUS+ shoots and T1 progeny contained T-DNA. Molecular characterization of one primary transformant and its T1 and T2 progeny plants established that T-DNA was integrated into the host genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baker CM, Wetzstein HY (1994) Plant Cell Tiss Org Cult (in press)

  • Beck E, Ludwig G, Awerswald EA, Reiss B, Schaller, H (1982) Gene 19:324–336

    Google Scholar 

  • Boote KJ (1982) Peanut Sci 9:35–40

    Google Scholar 

  • Brar GS, Cohen BA, Vick CL, Johnson GW (1994) The Plant J 5:745–753

    Google Scholar 

  • Byrne MC, McDonnell RE, Wright MS, Carnes MG (1987) Plant Cell Tiss Org Cult 8:3–15

    Google Scholar 

  • Chee PP, Drong RF, Slightom JL (1991) In: Gelvin S, Shapiro R (eds) Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht The Netherlands, pp 1–28

    Google Scholar 

  • Chee PP, Fober KA, Slightom JL (1989) Plant Physiol 91:1212–1218

    CAS  Google Scholar 

  • Clemente TE, Robertson D, Isleib TG, Beute MK, Weissinger AK (1992) Transgen Res 1:275–284

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol Biol Rep 1:19–21

    CAS  Google Scholar 

  • Feldmann KA, Marks MD (1987) Mol Gen Genet 208:1–9

    Google Scholar 

  • Franklin CI, Shorrosh KM, Trieu AN, Cassidy BG, Nelson RS (1993) Transgen Res 2:321–324

    Google Scholar 

  • Hobbs SLA, Jackson JA, Manon JD (1989) Plant Cell Rep 8:274–277

    Google Scholar 

  • Hood EE, Chilton WS, Chilton MD, Fraley RT (1986a) J Bacteriol 168:1283–1290

    Google Scholar 

  • Hood EE, Helmer GL, Fraley RT, Chilton MD (1986b) J Bacteriol 168:1291–1301

    CAS  PubMed  Google Scholar 

  • Hood EE, Jen G, Kayes L, Kramer J, Fraley RT, Chilton MD (1984) Bio/Technology 2:702–708

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Jefferson RA, Burgess SM, Hirsch D (1986) Proc Natl Acad Sci USA 83:8447–8451

    CAS  PubMed  Google Scholar 

  • Knauft DA, Gorbet DW (1989) Crop Sci 29:1417–1422

    Google Scholar 

  • Lacorte C, Mansur E, Timmerman B, Cordeiro AR (1991) Plant Cell Rep 10:354–357

    Google Scholar 

  • Mansur EA, Lacorte C, Freitas VGD, Oliveira DED, Timmerman B, Cordeiro AR (1993) Plant Sci 89:93–99

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Ozias-Akins P, Anderson WF, Holbrook CC (1992) Plant Sci 83:103–111

    Google Scholar 

  • Ozias-Akins P, Schnall JA, Anderson WF, Singsit C, Clemente TE, Adang MJ, Weissinger AK (1993) Plant Sci 93:185–194

    Google Scholar 

  • Rogers SG, Klee HJ, Horsch RB, Fraley RT (1987) Meth Enzymol 153:253–277

    Google Scholar 

  • Sellars RM, Southward GM, Phillips GC (1990) Crop Sci 30:408–414

    Google Scholar 

  • Southern EM (1975) J Mol Biol 98:503–517

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Land, Epcot, P.O. Box 10,000, 32830, Lake Buena Vista, FL, USA

    A. H. McKently

  2. Horticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, 32611, Gainesville, FL, USA

    G. A. Moore

  3. USDA/ARS Horticultural Research Laboratory, 32803, Orlando, FL, USA

    H. Doostdar & R. P. Niedz

Authors
  1. A. H. McKently
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. A. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Doostdar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. P. Niedz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G.C. Phillips

Rights and permissions

Reprints and permissions

About this article

Cite this article

McKently, A.H., Moore, G.A., Doostdar, H. et al. Agrobacterium-mediated transformation of peanut (Arachis hypogaea L.) embryo axes and the development of transgenic plants. Plant Cell Reports 14, 699–703 (1995). https://doi.org/10.1007/BF00232650

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232650

Keywords

Search

Navigation