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Regeneration of plants from protoplasts of rapid cycling Brassica oleracea L.

Plant Cell Reports volume 13, pages 335–339 (1994)Cite this article

Abstract

Rapid cycling Brassica species have great potential in plant genetic research because of their short life cycles and their minimal space requirements. Rapid cycling B. oleracea can be grown with up to six generations per year. Protoplast culture of this genotype can be applied for gene transfer by direct DNA uptake and by protoplast fusion. We here report on fast regeneration of flowering plants from protoplasts of rapid cycling B. oleracea. Regeneration frequencies of 27–65% were achieved with multiple shoots developing from individual calli. The regenerated plants were grown to maturity, and flowering and other morphological characteristics were monitored. The regenerants flowered within a similar time frame as plants grown from seeds. The ploidy level of regenerated and seed-grown plants was measured by flow cytometry. Many (20–45%) of the regenerants were tetraploid. Although only few seeds could be obtained from the tetraploids, large numbers of seeds with good germination were recovered from the diploid regenerants.

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Abbreviations

MS-3,0:

Murashige and Skoog medium containing 3% sucrose and no growth regulators

MES:

1-morpholino-ethane sulfonate

References

  • Arumuganathan K, Earle ED (1991a) Plant Mol Bio Rep 9: 208–218

    Google Scholar 

  • Arumuganathan K, Earle ED (1991b) Plant Mol Bio Rep 9: 221–231

    Google Scholar 

  • Delpierre N, Boccon-Gibod J (1992) Plant Cell Reports 11: 351–354

    Google Scholar 

  • Fu Y, Jia S, Lin Y (1985) Theor Appl Genet 71: 495–499

    Google Scholar 

  • Glimelius K (1984) Physiol Plant 61: 38–44

    Google Scholar 

  • Jourdan PS, Earle ED (1989) J Amer Soc Hort Sci 114: 343–349

    Google Scholar 

  • Kao HM, Keller WA, Gleddie S, Brown GG (1990) Plant Cell Reports 9: 311–315

    Google Scholar 

  • Koda T, Ichi T, Yamagishi H, Yoshikawa H (1988) Agric Biol Chem 52: 2337–2340

    Google Scholar 

  • Lentini Z, Christey M, Earle ED, Mutschler MA (1986) Eucarpia Cruciferae Newsletter 11: 86–87

    Google Scholar 

  • Loudon PT, Nelson RS, Ingram DS (1989) Plant Cell, Tissue and Organ Culture 19: 213–224

    Google Scholar 

  • May AW, Bosland PW, Williams PH (1986) Third Crucifer Genetics Workshop Proceedings. 50 [Abstr]

  • Menczel L, Nagy F, Kiss ZS, Maliga P (1981) Theor Appl Genet 59: 191–195

    Google Scholar 

  • Monteiro AA, Gabelman WH, Williams PH (1988) Eucarpia Cruciferae Newsletter 13: 122–123

    Google Scholar 

  • Mukhopadhyay A, Töpfer R, Pradhan AK, Sodhi YS, Steinbiß H-H, Schell J, Pental D (1991) Plant Cell Reports 10: 375–379

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 473–497

    CAS  Google Scholar 

  • Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rouselle P, Renard M (1983) Mol Gen Genet 191: 244–250

    Google Scholar 

  • Robertson D, Earle ED (1986) Plant Cell Reports 5: 61–64

    Google Scholar 

  • Rood SB, Pearce D, Williams PH, Pharis RP (1989) Plant Physiology 89: 482–487

    Google Scholar 

  • Tang K, Williams PH (1988) Eucarpia Cruciferae Newsletter 13: 59

    Google Scholar 

  • Toriyama K (1992) In: K Oono, T Hirabayashi, S Kikuchi, H Handa, K Kajiwara (eds) Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals. NIAR, Japan, pp 165–171

    Google Scholar 

  • Walters TW, Earle ED (1990) Plant Cell Reports 9: 316–319

    Google Scholar 

  • Williams P (1985) CrGC Resource Book, Dept of Plant Pathology, Univ of Wisconsin-Madison

  • Williams PH, Hill CB (1986) Science 232: 1385–1389

    Google Scholar 

  • Xu Z-H, Davey MR, Cocking EC (1982) Plant Science Letters 24: 117–121

    Google Scholar 

  • Yamashita H, Araki H, Yakuwa T (1991) J Fac Agr Hokkaido Univ 65: 119–125

    Google Scholar 

  • Ye G-N, Earle ED (1991) Plant Cell Reports 10: 213–216

    Google Scholar 

Download references

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Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA

    Lise N. Hansen & Elizabeth D. Earle

Authors
  1. Lise N. Hansen
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  2. Elizabeth D. Earle
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Communicated by C. F. Quiros

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Hansen, L.N., Earle, E.D. Regeneration of plants from protoplasts of rapid cycling Brassica oleracea L.. Plant Cell Reports 13, 335–339 (1994). https://doi.org/10.1007/BF00232632

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  • DOI: https://doi.org/10.1007/BF00232632

Keywords

  • Ploidy Level
  • Multiple Shoot
  • Brassica Oleracea
  • Protoplast Fusion
  • Space Requirement