Summary
Using a mathematical model of carbohydrate metabolism in Dictyostelium discoideum, the kinetic expressions describing the activities of glucokinase and glucose-6-P phosphatase have been analyzed. The constraints on the kinetic mechanisms and relative activities of these two enzymes were investigated by comparing computer simulations to experimental data. The results indicated that, (1) glucose-6-P is compartmentalized with respect to the enzymes involved in glucose-6-P, trehalose and glycogen metabolism, (2) a differences of approximately 0.6 mm/min in maximum specific activity of glucokinase compared to glucose-6-P phosphatase is required in order for the model to produce end product carbohydrate levels consistent with those observed experimentally, (3) the Km of glucokinase for glucose strongly influences the steady state levels of glucose in the absence of external glucose, and (4) changing the order of product removal in the reaction catalyzed by glucose-6-P phosphatase influences the level of glycogen and trehalose.
Similar content being viewed by others
References
Wright, B.E., 1973. Critical Variables in Differentiation. Prentice-Hall, Inc., Englewood Cliffs, New Jersey.
Wright, B. E. and Gustafson, G. L., 1972. J. Biol. Chem. 247, 7875–7884.
Wright, B. E., Tai, A. and Killick, K. A., 1977. Eur. J. Biochem. 74, 217–225.
Park, D. J. M. and Wright, B. E., 1975. Comput. Programs Biomed. 3, 10–26.
Wright, B. E., 1975. In: Microbiology 1975, p. 500–507, (Am. Soc. Microbiol.).
Cleland, W. W., 1963. Biochim. Biophys. Acta. 67, 104–137.
Baumann, P., 1969. Biochemistry 8, 5011–5015.
Killick, K. A. and Wright, B. E., 1978. J. Bacteriol., in press.
Noat, G., Richard, J., Borel, M. and Got, C., 1968. Eur. J. Biochem. 5, 55–70.
Gezelius, K., 1966. Physiol. Plant 19, 946–959.
Gezelius, K. and Wright, B. E., 1965. J. Gen. Microbiol. 38, 309–327.
Lueck, J. D., Herrman, J. L. and Nordlie, R. C., 1972. Biochemistry 11, 2792–2799.
Wright, B. E. and Bloom, B., 1960. Biochim. Biophys. Acta 48, 342–346.
Purich, D. L. and Fromm, H. J., 1972. In: Current Topics in Cellular Regulation, Vol. 6, 131–166. Ed. B. L. Horecker and E. R. Stadtman, Acad. Press, Inc.
Savageau, M., 1976. Biochemical Systems Analysis, Addison Wesley, Inc., Reading, Massachusetts.
Cleland, W. W., 1967. Ann. Rev. Biochem. 36, 77–112.
Gots, R. E. and Bessman, S. P., 1974. Arch. Biochem. Biophys. 163, 7–14.
Risse, J. J. and Blum, L. J., 1972. Arch. Biochem. Biophys. 149, 329–335.
Weiner, E. and Ashworth, J. M., 1970. Biochem. J. 118, 505–512.
Srere, P. and Mosbach, 1974. Ann. Rev. Microbiol. 61–83.
Williamson, D. H., Lund, P. and Krebs, H. A., 1967. Biochem. J. 103, 514–527.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kelleher, J.K., Kelly, P.J. & Wright, B.E. A kinetic analysis of glucokinase and glucose-6-P phosphatase in dictyostelium. Mol Cell Biochem 19, 67–73 (1978). https://doi.org/10.1007/BF00232596
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00232596