Skip to main content
SpringerLink
Log in

The stimulation of ion fluxes in brain slices by glutamate and other excitatory amino acids

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

The stimulation of ion movements by excitatory amino acids in brain slices allows the study of various events related to the process of excitatory neurotransmission. Presynaptic mechanisms of uptake of putative neurotransmitters can be followed by the influx of Na+ ions. Postsynaptic depolarizations due to the activation of action potentials or of ionophores associated with specific receptors can be monitored by measurements of the rate of efflux of radioactive tracer ions. Thus, the pharmacological properties of the excitatory amino acid receptors can be investigated as well as those of their putative endogenous effectors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Terner, C., Eggleston, L. V. & Krebs, H. A., 1950. Biochem. J. 47: 139–149.

    Google Scholar 

  2. Hayashi, T., 1954. Keio J. Med. 3: 183–192.

    Google Scholar 

  3. Curtis, D. R., Phillis, J. W. & Watkins, J. C., 1959. Nature 183: 611–612.

    PubMed  Google Scholar 

  4. Curtis, D. R., Phillis, J. W. & Watkins, J. C., 1960. J. Physiol. 150: 656–682.

    PubMed  Google Scholar 

  5. Curtis, D. R. & Watkins, J. C., 1965. Pharmacol. Rev. 17: 347–391.

    PubMed  Google Scholar 

  6. Bradford, H. F. & McIlwain, H., 1966. J. Neurochem. 13: 1163–1177.

    PubMed  Google Scholar 

  7. Bennett, J. P. Jr., Logan, W. J. & Snyder, S. H., 1973. J. Neurochem. 21: 1533–1550.

    PubMed  Google Scholar 

  8. Campbell, G. Lem. & Shank, R. P., 1978. Brain Res. 153: 618–622.

    PubMed  Google Scholar 

  9. Curtis, D. R. & Johnston, G. A. R., 1974. Ergebn. der. Physiol. 69: 97–188.

    Google Scholar 

  10. Stallcup, W. B., Bulloch, K. & Baetge, E. E., 1979. J. Neurochem. 32: 57–65.

    PubMed  Google Scholar 

  11. Balcar, V. J. & Johnston, G. A. R., 1972. J. Neurobiol. 3: 295–301.

    PubMed  Google Scholar 

  12. Johnston, G. A. R., Kennedy, S. M. & Twitchin, B., 1979. J. Neurochem. 32: 121–127.

    PubMed  Google Scholar 

  13. Roberts, P. J. & Watkins, J. C., 1975. Brain Res. 85: 120–125.

    PubMed  Google Scholar 

  14. Shank, R. P. & Aprison, M. H., 1979. Glutamic Acid: Advances in Biochemistry and Physiology (Filer, L. J. Jr., Garattini, S., Kare, M. R., Reynolds, W. A. & Wurtman, R. J., eds.), pp. 139–150. Raven Press, New York.

  15. Krnjevic, K. & Schwartz, S., 1967. Exp. Brain Res. 3: 306–319.

    PubMed  Google Scholar 

  16. Zieglgänsberger, W. & Puil, E. A., 1973. Exp. Brain Res. 17: 35–49.

    PubMed  Google Scholar 

  17. Hösli, L. & Hösli, E., 1978. Rev. Physiol. Biochem. Pharmacol. 81: 136–188.

    Google Scholar 

  18. Harvey, J. A. & McIlwain, H., 1968. Biochem. J. 108: 269–274.

    PubMed  Google Scholar 

  19. McIlwain, H., Harvey, J. A. & Rodriguez, G., 1969. J. Neurochem. 16, 363–370.

    PubMed  Google Scholar 

  20. Pull, I., McIlwain, H. & Ramsey, R. L., 1970. Biochem. J. 116: 181–187.

    PubMed  Google Scholar 

  21. Biziere, K. & Coyle, J. T., 1978. J. Neurochem. 31: 513–520.

    PubMed  Google Scholar 

  22. Kasai, M. & Changeux, J. P., 1971. J. Memb. Biol. 6: 1–23.

    Google Scholar 

  23. Catterall, W. A., 1975. J. Biol. Chem. 250: 1776–1781.

    PubMed  Google Scholar 

  24. Ticku, M. K. & Olsen, R. W., 1977. Biochim. biophys. Acta 464: 519–529.

    PubMed  Google Scholar 

  25. Manger, J. P., Moura, A. M. & Worcel, M., 1978. Br. J. Pharmacol. 64: 29–36.

    PubMed  Google Scholar 

  26. Catterall, W. A. & Nirenberg, M., 1973. Proc. Nat. Acad. Sci. USA 70: 3759–3763.

    PubMed  Google Scholar 

  27. Catterall, W. A., 1975. Proc. Nat. Acad. Sci. USA 72: 1782–1786.

    PubMed  Google Scholar 

  28. Stallcup, W. B., 1977. Brain Res. 135: 37–53.

    PubMed  Google Scholar 

  29. Palfrey, C. & Littauer, U. Z., 1976. Biochem. Biophys. Res. Common. 72: 209–215.

    Google Scholar 

  30. Stallcup, W. B., 1979. J. Physiol. 286: 525–540.

    PubMed  Google Scholar 

  31. Charnock, J. S., Cook, D. A. & Casey, R., 1971. Arch. Biochem. Biophys. 147: 323–329.

    PubMed  Google Scholar 

  32. Katz, B., 1966. ‘Nerve Muscle and Synapse’, McGraw Hill, New York.

    Google Scholar 

  33. Teichberg, V. L, Goldberg, O., Tal, N. & Luini, A., 1980. Neurotransmitters and their Receptors (Littauer, U. Z., Dudai, Y., Silman, I., Teichberg, V. I. & Vogel, Z., ed.), pp. 349–368, John Wiley, N. Y.

  34. Yamamoto, C., Yamashita, H. & Chujo, T., 1976. Nature 262: 786–787.

    PubMed  Google Scholar 

  35. Krnjevic, K. & Phillis, J. W., 1963. J. Physiol. 165: 274–304.

    PubMed  Google Scholar 

  36. Ransom, B. R., Bullock, P. N. & Nelson, P. G., 1977. J. Neurophysiol. 40: 1163–1177.

    PubMed  Google Scholar 

  37. Daniel, E. E., 1975. Methods in Pharmacology 3: 699–721.

    Google Scholar 

  38. Curtis, D. R., Duggan, A. W., Felix, D., Johnston, G. A. R., Tebecis, A. K. & Watkins, J. C., 1972. Brain Res. 41: 283–301.

    PubMed  Google Scholar 

  39. Goldberg, O., Luini, A. & Teichberg, V. I. 1980. Tetrahedron Lett. 21: 2355–2358.

    Google Scholar 

  40. Nathanson, J. A., 1977. Physiol. Rev. 57, 157–256.

    PubMed  Google Scholar 

  41. Greengard, P., 1976. Nature, 260: 101–108.

    PubMed  Google Scholar 

  42. Shimizu, H., Ichishita, H. & Odagiri, H., 1974. J. Biol. Chem. 249: 5955–5962.

    PubMed  Google Scholar 

  43. Ferrendelli, J. A., Chang, M. M. & Kinscherf, D. A., 1974. J. Neurochem. 22, 535–540.

    PubMed  Google Scholar 

  44. Mao, C. C., Guidotti, A. & Costa, A., 1974. Brain Res. 79: 510–514.

    PubMed  Google Scholar 

  45. Garthwaite, J. & Balazs, R., 1978. Nature 275, 328–329.

    PubMed  Google Scholar 

  46. Crank, J., 1956. The Mathematics of Diffusion, Oxford at the Clerendon Press.

  47. Nicholson, C., Phillips, J. M. & Gardner-Medwin, A. R., 1979. Brain Res. 169: 580–584.

    PubMed  Google Scholar 

  48. Biziere, K., Thompson, H. & Coyle, J. T., 1980. Brain Res. 183: 421–423.

    PubMed  Google Scholar 

  49. Baudry, M. & Lynch, G., 1980. Proc. Nat. Acad. Sci. USA 77, 2298–2302.

    PubMed  Google Scholar 

  50. Foster, A. C. & Roberts, P. J., 1978. J. Neurochem. 31: 1467–1777.

    PubMed  Google Scholar 

  51. Davies, J. & Watkins, J. C., 1979. J. Physiol. 297: 621–635.

    PubMed  Google Scholar 

  52. McLennan, H. & Lodge, D., 1979. Brain Res. 169: 83–90.

    PubMed  Google Scholar 

  53. Watkins, J. C., 1980. Trends in Neurosciences 3: 61–64.

    Google Scholar 

  54. Davies, J., Evans, R. H., Francis, A. A., Jones, A. W. & Watkins, J. C., 1980. Neurotransmitters and their Receptors (Littauer, U. Z., Dudai, Y., Silman, I., Teichberg, V. I. & Vogel, Z. ed.), pp. 333–348, John Wiley, N.Y.

  55. Goldberg, O., Luini, A. & Teichberg, V. I., 1981. Neuroscience Lett. (in press).

  56. Hall, J. G., Hicks, T. P. & McLennan, H., 1978. Neuroscience Lett. 8, 171–175.

    Google Scholar 

  57. McLennan, H. & Lodge, D., 1979. Brain Res. 169: 83–90.

    PubMed  Google Scholar 

  58. Segal, M., 1976. Br. J. Pharmac. 58: 341–345.

    Google Scholar 

  59. Spencer, H. J., Gribkoff, V. K., Cotman, C. W. & Lynch, G., 1976. Brain Res. 105: 471–481.

    PubMed  Google Scholar 

  60. Clarke, G. & Straughan, D. W., 1977. Neuropharmacology 16: 391–398.

    PubMed  Google Scholar 

  61. McGeer, P. L., McGeer, E. G., Scherer, U. & Singh, K., 1977. Brain Res. 128: 369–373.

    PubMed  Google Scholar 

  62. Reubi, J. C. & Cuenod, M., 1979. Brain Res. 176: 185–188.

    PubMed  Google Scholar 

  63. Rowlands, G. J. & Roberts, P. J., 1980. Exp. Brain Res. 39: 239–240.

    PubMed  Google Scholar 

  64. Spencer, H. J., 1976. Brain Res. 102: 91–101.

    PubMed  Google Scholar 

  65. Evans, R. H., Francis, A. A., Hunt, K., Oakes, D. J. & Watkins, J. C., 1979. Br. J. Pharmacol. 67: 591–603.

    PubMed  Google Scholar 

  66. Biscoe, T. J., Davies, J., Dray, A., Evans, R. H., Francis, A. A., Martin, M. R. & Watkins, J. C., 1977. Europ. J. Pharmacol. 54: 315–316.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Neurobiology, The Weizmann Institute of Science, Rehovot, Israel

    Vivian I. Teichberg & Alberto Luini

  2. Dept. of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel

    Ora Goldberg

Authors
  1. Vivian I. Teichberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ora Goldberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alberto Luini
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Teichberg, V.I., Goldberg, O. & Luini, A. The stimulation of ion fluxes in brain slices by glutamate and other excitatory amino acids. Mol Cell Biochem 39, 281–295 (1981). https://doi.org/10.1007/BF00232580

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232580

Keywords

Search

Navigation