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Expression of an abscisic acid responsive promoter in Picea abies (L.) Karst. following bombardment from an electric discharge particle accelerator

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Abstract

The 1.5 kilobase promoter sequence upstream of Dc8, a late embryo abundant gene of Daucus, fused to the reporter β-glucuronidase gene was introduced into several tissues of Picea abies via a custom-made electric-discharge particle accelerator. Transient expression was measured histochemically as spot number 2 d after bombardment. Embryogenic suspensions gave higher levels of expression depending upon cell line than embryogenic callus or zygotic embryos. Expression was enhanced when cultures were treated with abscisic acid for 3 d before bombardment. A mean and maximum of 17 and 34 spots/disk, respectively, were observed with the best cell line, which was comparable with the level of expression driven by an enhanced 35S promoter.

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Abbreviations

ABA:

Abscisic acid

BA:

Benzyladenine

2,4-D:

2, 4-dichlorophenoxyacetic acid

References

  • Chang S, Sen S, McKinley CR, Aimers-Halliday J, Newton RJ (1991) Plant Cell Rep 10:131–134

    Google Scholar 

  • Christou P (1990) Physiol Plant 79:210–212

    Google Scholar 

  • Duchesne LC, Charest PJ (1991) Plant Cell Rep 10:191–194

    Google Scholar 

  • Ellis DD, McCabe D, Russell D, Martinell B, McCown BH (1991) Plant Molec Biol 17:19–27

    Google Scholar 

  • Good AG, Bekkaoui F, Pilate G, Dunstan DI, Crosby WL (1990) Physiol Plant 78:441–446

    Google Scholar 

  • Guerineau F, Brooks L, Meadows J, Lucy A, Robinson C, Mullineaux P (1990) Plant Mol Biol 15:127–136

    Google Scholar 

  • Gupta PK, Dandekar AM, Durzan DJ (1988) Plant Sci 58:85–92

    Google Scholar 

  • Hakman I, von Anold S (1988) Physiol Plant 72:579–587

    Google Scholar 

  • Hatzopoulos P, Fong F, Sung ZR (1990) Plant Physiol 94:690–695

    CAS  Google Scholar 

  • Jalonen P, von Arnold S (1991) Plant Cell Rep 10:384–387

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Klein TM, Harper EC, Svab Z, Sanford JC, Fromm ME, Maliga P (1988) Proc Natl Acad Sci USA 85:8502–8505

    Google Scholar 

  • Loopstra CA, Sederoff RR (1990) IUFRO Molecular Genetics Working Party Meeting Stanford Sierra Club, Fallen Leaf Lake, Lake Tahoe, CA Sept 30–Oct 4

  • Marcotte WR, Bayley CC, Quatrano RS (1988) Nature 335:454–457

    Google Scholar 

  • McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Bio/Technology 6:923–926

    Google Scholar 

  • Mo LH, von Arnold S (1991) Origin and development of embryonic cultures from seedlings of Norway spruce (Picea abies) J Plant Physiol 138:223–230

    Google Scholar 

  • Seffens WS, Almoguera C, Wilde HD, Voner Haar RA, Thomas TL (1990) Dev Gen 11:65–76

    CAS  PubMed  Google Scholar 

  • Sen S, Newton RJ, Fong F, Neuman P (1989) Plant Cell Rep 8:191- 194

    Google Scholar 

  • Stomp A-M, Weissinger A, Sederoff RR (1991) Plant Cell Rep 10:187–190

    Google Scholar 

  • Tautorus TE, Bekkaoui F, Pilon M, Datla RSS, Crosby WL, Fowke LC, Dunstan DI (1989) Theor Appl Genet 78:531–536

    Google Scholar 

  • von Arnold S (1987) J Plant Physiol 128:233–244

    Google Scholar 

  • von Arnold S, Hakman I (1988a) J Plant Physiol 132:164–169

    Google Scholar 

  • von Arnold S, Hakman I (988b) In: JW Hanover and DE Keathley, eds Genetic Manipulation of Woody Plants Plenum Pub Corp pp 199–215

  • Wilson S, Thorpe T, Maloney M (1989) Plant Cell Rep 7:704–707

    Google Scholar 

  • Yibrah HS, Clapham DH (1990) IUFRO Molecular Genetics Working Party Meeting Stanford Sierra Club Fallen Leaf Lake Lake Tahoe, CA Sept 30–Oct 4

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Authors and Affiliations

  1. Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX

    Ronald J. Newton & Niu Dong

  2. Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden

    Haile S. Yibrah, David H. Clapham & Sara von Arnold

Authors
  1. Ronald J. Newton
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  2. Haile S. Yibrah
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  3. Niu Dong
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  4. David H. Clapham
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  5. Sara von Arnold
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Communicated by G. C. Phillips

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Newton, R.J., Yibrah, H.S., Dong, N. et al. Expression of an abscisic acid responsive promoter in Picea abies (L.) Karst. following bombardment from an electric discharge particle accelerator. Plant Cell Reports 11, 188–191 (1992). https://doi.org/10.1007/BF00232530

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  • DOI: https://doi.org/10.1007/BF00232530

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