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Polymerase chain reaction comparison of the gene for strictosidine synthase from ten Rauvolfia species

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Abstract

The gene for strictosidine synthase, str1, has been analyzed by the polymerase chain reaction in ten species of Rauvolfia, the origins of which span the tropical belt: R. cambodiana (Indochina), R. canescens (India), R. chinensis (China), R. heterophylla (Central America), R. mannii (West Africa), R. nitida (West Indies), R. praecox (Brasil), R. serpentina (India), R. sumatrana (Indonesia) and R. verticillata (Indochina). Restriction endonuclease analysis of the gene fragments produced with genomic DNA from each of the ten species as template revealed that str1 is highly conserved in the Rauvolfia species investigated. These results suggest that there is a stringent selection pressure on the gene for this key enzyme of indole alkaloid biosynthesis.

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Abbreviations

PCR:

polymerase chain reaction

References

  • Bracher D, Kutchan TM (1992) Arch Biochem Biophys, in press

  • Hamill JD, Rounsley S, Spencer A, Todd G, Rhodes MJC (1991) Plant Cell Reports 10: 221–224

    Google Scholar 

  • Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) (1990) PCR Protocols, Academic Press, San Diego

    Google Scholar 

  • Kutchan TM (1989) FEBS Lett 257: 127–130

    Google Scholar 

  • Kutchan TM, Dittrich H, Bracher D, Zenk MH (1991) Tetrahedron 47: 5945–5954

    Google Scholar 

  • Kutchan TM, Hampp N, Lottspeich F, Beyreuther K, Zenk MH (1988) FEBS Lett 237: 40–44

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Physiol Plant 18: 100–127

    CAS  Google Scholar 

  • McKnight TD, Roessner CA, Devagupta R, Scott AI, Nessler CL (1990) Nucl Adds Res 18: 4939

    Google Scholar 

  • Smith GN (1968) Chem Commun 912–914

  • Southern E (1980) J Mol Biol 98: 503–517

    Google Scholar 

  • Stöckigt J, Zenk MH (1977a) Chem Commun 646–648

  • Stöckigt J, Zenk MH (1977b) FEBS Lett 79: 233–237

    Google Scholar 

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Authors and Affiliations

  1. Lehrstuhl für Pharmazeutische Biologie, Universität München, Karlstraße 29, 2, München, Germany

    Daniel Bracher & Toni M. Kutchan

Authors
  1. Daniel Bracher
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  2. Toni M. Kutchan
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Communicated by N. Amrhein

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Bracher, D., Kutchan, T.M. Polymerase chain reaction comparison of the gene for strictosidine synthase from ten Rauvolfia species. Plant Cell Reports 11, 179–182 (1992). https://doi.org/10.1007/BF00232528

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  • DOI: https://doi.org/10.1007/BF00232528

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