Summary
Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (−196°C) exposure following a vitrification-inducing pretreatment. Shoot tips were treated in a stepwise manner with a vitrification solution containing 30% glycerol, 15% ethylene glycol and 15% dimethylsulfoxide in growth medium. Incubation of shoot tips for 1 to 2 h in low concentrations of the vitrification solution enhanced survival. Most surviving shoot tips developed callus, and a variable percentage subsequently formed shoots. Survival was not achieved using two-step cooling procedures. The percentage of shoot tips surviving vitrification and those subsequently forming a shoot varied widely among replications.
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Abbreviations
- BA:
-
N6-benzyladenine
- IBA:
-
indole-3-butyric acid
- EG:
-
ethylene glycol
- DMSO:
-
dimethylsulfoxide
- MS:
-
Murashige and Skoog (1962) minerals and vitamins
- LN:
-
liquid nitrogen
- PI:
-
plant introduction
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Communicated by G. C. Phillips
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Towill, L.E., Jarret, R.L. Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification. Plant Cell Reports 11, 175–178 (1992). https://doi.org/10.1007/BF00232527
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DOI: https://doi.org/10.1007/BF00232527