Skip to main content
SpringerLink
Log in

Reduction of 2,3,5-triphenyltetrazolium chloride by the KCN-insensitive, salicylhydroxamic acid-sensitive alternative respiratory pathway of mitochondria from cultured grapevine cells

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by grapevine cells cultured in suspension was studied in order to assess the reliability of using TTC reduction as a measure of cell viability. Similar to the reduction observed in animals cells, TTC can be reduced in grapevine cells by the cytochrome respiratory path of the mitochondria, although it is mostly reduced (about 72 %) by the alternative respiratory path sensitive to salicylhydroxamic acid. Engagement of the alternative path in TTC reduction was calculated through the √Valt plot, and was established to be 89 %.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

SHAM:

salicylhydroxamic acid

TTC:

2,3,5-triphenyltetrazolium chloride

References

  • Bahr JT, Bonner WD (1973) J Biol Chem 248: 3441–3445

    Google Scholar 

  • Bingham IJ, Farrar JF (1987) Physiol Plant 70: 491–498

    Google Scholar 

  • Day DA, Arron GP, Laties GG (1980) In: Davies DD (ed) The Biochemistry of Plants, Vol. 2, Academic Press, New York, pp 197–241

    Google Scholar 

  • Gamborg OL, Miller RA, Ojika K (1968) Exp Cell Res 50: 151–158

    CAS  PubMed  Google Scholar 

  • Huang CN, Cornejo MJ, Bush DS, Jones RL (1986) Protoplasma 135: 80–87

    Google Scholar 

  • Lambers H (1982) Physiol Plant 55: 478–485

    CAS  Google Scholar 

  • Laties GG (1982) Ann Rev Plant Physiol 33: 519–555

    Google Scholar 

  • Miller IM, Berczi A, van der Plas LHW, Lambers H (1988) Physiol Plant 72: 642–649

    Google Scholar 

  • Möllering H, Wahlefeld AW, Michal G (1974) In: Bergmeyer HU, Gawehn K (eds) Methods of Enzymatic Analysis, Vol. 1, Academic Press, New York, pp 136–144

    Google Scholar 

  • Schonbaum GR, Bonner WD, Storey BT, Bahr JT (1971) Plant Physiol 47: 124–128

    Google Scholar 

  • Yokoyama M, Inomata S, Seto S, Yanagi M (1990) Plant Cell Physiol 31: 551–555

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Biology (Plant Physiology), University of Murcia, Campus of Espinardo, E-30071, Murcia, Spain

    J. M. Zapata, C. Salinas, A. A. Calderón, R. Muñoz & A. Ros Barceló

Authors
  1. J. M. Zapata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Salinas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Calderón
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Muñoz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Ros Barceló
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by A. Boudet

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zapata, J.M., Salinas, C., Calderón, A.A. et al. Reduction of 2,3,5-triphenyltetrazolium chloride by the KCN-insensitive, salicylhydroxamic acid-sensitive alternative respiratory pathway of mitochondria from cultured grapevine cells. Plant Cell Reports 10, 579–582 (1991). https://doi.org/10.1007/BF00232515

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232515

Keywords

Search

Navigation