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Pathogenesis-related proteins in lima bean leaves infected with tobacco ringspot virus and their distribution within and around local lesions

Plant Cell Reports volume 12, pages 55–59 (1992)Cite this article

Abstract

Tobacco ringspot virus (TRSV) induces circular, darkbrown local lesions on primary leaves of lima bean (Phaseolus lunatus cv Nemagreen) with a concomitant production of three basic and three acidic pathogenesisrelated (PR) proteins. The three basic proteins are: a 21 kDa protein related serologically to Pinto bean PR-4d and tobacco PR-5 proteins; a 36 kDa glucanase that is related to tobacco PR-2; and, a 31 kDa chitinase related serologically to ethylene-induced bean chitinase. The three acidic 18 kDa lima bean PR proteins are serologically similar and probably are charged isomers of the same protein. The 21 kDa basic protein and the 18 kDa acidic protein accumulated preferentially at the lesion center while the 31 kDa chitinase and TRSV were distributed evenly throughout the necrotic area. In green tissue immediately surrounding a lesion, the amounts of PR proteins were comparable to or lower than those in the necrotic area, and virions were not detected. This mode of spatial distribution indicates that lima bean PR proteins are not involved in TRSV localization, and is consistent with other observations that PR proteins play no direct role in restricting viral spread.

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References

  • Abeles FB, Bosshart RP, Florence LE, Habig WH (1970) Plant Physiol 47:129–134.

    Google Scholar 

  • Antoniw JF, White RF (1986) Plant Mol. Biol. 6:145–149

    Google Scholar 

  • Antoniw JF, Ritter CE, Pierpoint WS, Van Loon LC (1980) J Gen Virol 47:79–87.

    Google Scholar 

  • Awade A, De Tapia M, Didierjean L, Burkhard G. (1989) Plant Sci 63:121–130.

    Google Scholar 

  • Bol, JF, Linthorst HJM, Cornelissen BJC (1990) Annu Rev Phytopathol 28:113–128.

    Google Scholar 

  • Boller, JF, Gehri A, Mauch F, Vogeli U (1983) Planta 157:22–31.

    Google Scholar 

  • Cusack M, Pierpoint WP (1988) Phytochemistry 27:3817–1821.

    Google Scholar 

  • Cutt JR, Harpster MH, Dixon DC, Carr JP, Dunsmir P, Klessig D.F. (1989) Virology 173:89–97.

    Google Scholar 

  • De Tapia M, Bergmann P, Awade A, Burkhard G (1986) Plant Sci 45:167–177.

    Google Scholar 

  • Dumas E, Gianinazi S. (1986) Physiol Molec Plant Pathol 28:243–250.

    Google Scholar 

  • Kaufmann S, Legrand M, Geoffroy P, Fritig B. (1987) EMBO J 11:3209–3212.

    Google Scholar 

  • Legrand M, Kaufmann S, Geoffroy P, Fritig B. (1987) Proc Natl Acad Sci USA 84:6750–6754.

    Google Scholar 

  • Linthorst HJM. (1991) Crtical Rev Plant Sci 10:123–150.

    Google Scholar 

  • Linthorst HJM, Meuwissen RLJ, Kaufmann S, Bol J. (1989) Plant Cell 1:285–291.

    Google Scholar 

  • Parent JG, Asselin A. (1987) Phytopathology 87:1122–1125.

    Google Scholar 

  • Pierpoint WS, Jackson PJ Evans RM (1990) Physiol Plant Pathol 36:325–338.

    Google Scholar 

  • Reisfeld RA, Lewis UJ, Williams DE (1962) Nature 195:281–283.

    Google Scholar 

  • Sehgal OP, Mohamed F. (1990) In: Mandahar CL (ed) Plant Viruses-Pathology vol 2 CRC Press Inc., Boca Raton, FL.

    Google Scholar 

  • Sehgal OP, Rieger R, Mohamed F, (1991) Phytopathology 81:215–219.

    Google Scholar 

  • Van Loon LC (1985) Plant Mol Biol 4:111–116.

    Google Scholar 

  • Vigers, AJ, Wiedermann S, Roberts WK, Legrand M, Selitrennikoff CP, Fritig B. (1992) Plant Science 83:155–161.

    Google Scholar 

  • Vogeli U, Meins F, Boller T (1988) Planta 174:364–372.

    Google Scholar 

Download references

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Affiliations

  1. Department of Plant Pathology, University of Missouri, 65211, Columbia, MO, USA

    O. P. Sehgal

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  1. O. P. Sehgal
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Communicated by R. N. Beachy

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Sehgal, O.P. Pathogenesis-related proteins in lima bean leaves infected with tobacco ringspot virus and their distribution within and around local lesions. Plant Cell Reports 12, 55–59 (1992). https://doi.org/10.1007/BF00232424

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  • DOI: https://doi.org/10.1007/BF00232424

Keywords

  • Chitinase
  • Basic Protein
  • Local Lesion
  • Necrotic Area
  • Primary Leaf