Summary
We present protocols for the regeneration of fertile plants from immature zygotic embryos of Arabidopsis thaliana ecotype “Zürich” either directly by multiple shoot formation from the apical region or indirectly by shoot induction from embryo derived calli. The regeneration efficiency by “multiple shooting” depended on the developmental stage of the cultured embryos and ranged from 15% for early heart shaped to 90% for early torpedo shaped and further developed embryos. 85% callus induction was achieved from embryos in the early torpedo shaped or a later stage of development. The efficiency of shoot induction from embryo derived calli varied between 25% and 75% in different experiments.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
6-benzylaminopurine
- CIM:
-
callus inducing medium
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- Gln:
-
glutamine
- IAA:
-
indole-3-acetic acid
- Kin:
-
kinetin
- NAA:
-
l-naphthaleneacetic acid
- 2ip:
-
2-isopentenylaminopurine
- Pro:
-
proline
- RIM:
-
root inducing medium
- SIM:
-
shoot inducing medium
References
Baus AD, Franzmann L, Meinke DW (1986) Theor Appl Genet 72: 577–586
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158
Hisajima S (1982) Biologia Plantarium 24: 235–238
Hu C, Wang P (1986) In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture, Macmillan Publishing Company, New York, pp 43–96
Karesch H, Bilang R, Potrykus I (1991) Plant Cell Reports 9: 575–578
Kerr T, Anderson DB (1944) Plant Physiology 19: 338–349
Lupotto E, Lusardi MC (1988) Maydica 33: 163–177
Meyerowitz EM (1989) Cell 56: 263–269
Monnier M (1976) Rev Cytol Biol Veg 39: 1–120
Monnier M (1990) In: Bhojwani SS (ed) Plant tissue culture: applications and limitations, Elsevier, Amsterdam Oxford New York Tokyo, pp 366–393
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Negrutiu I, Beeftink F, Jacobs M (1975) Plant Science Lettres 5: 293–304
Potrykus I (1990) Bio/Technology 8: 535–542
Raghavan V, Srivastava PS (1982) In: Johri BM (ed) Experimental embryology of vascular plants, Springer, Berlin Heidelberg New York, pp 195–230
Rietsema J, Satina S, Blakeslee AF (1953) Am J Bot 40: 538–545
Rijven AHGC (1952) Acta Bot Neerl 1: 157–200
Rijven AHGC (1956) Aust J Biol Sci 9: 511–527
Spangenberg G, Koop HU, Lichter R, Schweiger HG (1986) Physiol Plant 66: 1–8
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Kost, B., Potrykus, I. & Neuhaus, G. Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana . Plant Cell Reports 12, 50–54 (1992). https://doi.org/10.1007/BF00232423
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232423