Abstract
Protoplasts were isolated from leaves of glasshouse-grown plants of Centaurea cyanus and axenic shoot cultures of Senecio x hybridus. Upon culture, using modified MS-based media, protoplasts of both systems entered division to produce callus, followed by plant regeneration. Leaf protoplasts of Callistephus chinensis entered sustained division only following the preconditioning for 24h of peeled leaf tissues on agar-solidified MS-based medium. Protoplasts were also isolated from cell suspensions of C. chinensis and divided in MS-based or KM media. However, only leaf mesophyll protoplasts of Callistephus produced callus, which developed shoots.
The establishment of protoplast-to-plant protocols for these ornamental species has provided a basis for broadening their gene pools through somatic hybridisation.
Similar content being viewed by others
Abbreviations
- BAP:
-
6-benzylaminopurine
- NAA:
-
α-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog (1962)
- KM:
-
Kao and Michayluk (1975)
- g.f.wt.:
-
gram fresh weight
References
Al-Atabee JS, Power JB (1987) Plant Cell Rep 6: 414–416
Al-Atabee JS, Mulligan BJ, Power JB (1990) Plant Cell Rep 8: 517–520
Berry SF, Lu DY, Pental D, Cocking EC (1982) Z Pflanzenphysiol 108: 31–38
Binding H, Nehls R, Kock R, Finger J Mordhorst G (1981) Z Pflanzenphysiol 101: 119–130
Davey MR (1983) International Symposium: Proceedings 6th Int Protoplast Symp. Basel 19–29
Donn G (1977) Z Pflanzenphysiol 86: 66–75
Evans DA, Bravo JE (1983) Int Rev Cytol, Suppl 16: 33–53
Frearson EM, Power JB, Cocking EC (1973) Dev Biol 33: 130–137
Gilmour DM, Davey MR, Cocking EC (1989) Plant Cell Rep 8: 29–32
Kao KN (1977) Mol Gen Genet 150: 225–230
Kao KN, Michayluk MR (1975) Planta 126: 105–110
Larkin PJ (1976) Planta 128: 213–216
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Otsuka M (1986) J Agric Sci 41: 109–112
Power JB, Davey MR, McLellan M, Wilson D, (1989) Laboratory Manual: Plant Tissue Culture, University of Nottingham, Nottingham, UK
Saxena PK (1985) J Plant Physiol 119: 385–388
Shepard JF, Totten RE (1977) Plant Physiol 60: 313–316
Author information
Authors and Affiliations
Additional information
Communicated by I. Potrykus
Rights and permissions
About this article
Cite this article
Pillai, V., Davey, M.R. & Power, J.B. Plant regeneration from mesophyll protoplasts of Centaurea cyanus, Senecio x hybridus and Callistephus chinensis . Plant Cell Reports 9, 402–405 (1990). https://doi.org/10.1007/BF00232409
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232409