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Micropropagation of Phytolacca dodecandra through shoot-tip and nodal cultures

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Abstract

A procedure for micropropagation of endod (Phytolacca dodecandra) is described. BA at 0.44 μM produced 3.1 new shoots per expiant in six weeks using shoot tips. Nodal expiants, however, produced up to 4.7 shoots per explant on medium with 0.44 μM BA and 0.27 μM GA,. IBA at 0.49 μM induced 90% rooting with minimal callus. Plantlets were successfully transferred to the greenhouse and some staminate clones produced flowers after six months.

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Abbreviations

BA:

6 benzylaminopurine

kinetin:

6-furfurylaminopurine

2iP:

N6-(2-isopentyl)adenine

IAA:

indole-3-acetic acid

NAA:

1-naphthaleneacetic acid

GA3 :

Gibberellic acid

IBA:

indole-3-butyric acid

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Authors and Affiliations

  1. Department of Horticulture, Colorado State University, 80523, Fort Collins, CO, USA

    Tigst Demeke & Harrison G. Hughes

Authors
  1. Tigst Demeke
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  2. Harrison G. Hughes
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Communicated by J. M. Widholm

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Demeke, T., Hughes, H.G. Micropropagation of Phytolacca dodecandra through shoot-tip and nodal cultures. Plant Cell Reports 9, 390–392 (1990). https://doi.org/10.1007/BF00232406

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  • DOI: https://doi.org/10.1007/BF00232406

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