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Selection of penstemide and serrulatoloside producing clones in penstemon serrulatus by small-aggregate cloning

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Abstract

A plating method is described for cultured cells of Penstemon serrulatus Menz. which allows effective cloning of aggregates consisting of less than 10 cells, on a simple synthetic medium. The obtained clones snowed high diversity with respect to their ability to produce penstemide, an ester iridoid of the Valeriana-type. Some of the clones produced higher quantities of this compound than the original cell line. The content of another iridoid glucoside of the same type, serrulatoloside, was also determined.

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Abbreviations

SH:

Schenk and Hildebrandt (1972)

IBA:

indole-3-butyric acid

BAP:

6-benzylaminopurine

PE:

plating efficiency

d.w.:

dry weight

sd:

standard deviation

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Authors and Affiliations

  1. Department of Botany, Institute of Environmental Research and Bioanalysis Medical Academy, Muszynskiego 1, PL-90-151, Łódź, Poland

    Halina Wysokińska & Nguyen Thi Xuan Giang

Authors
  1. Halina Wysokińska
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  2. Nguyen Thi Xuan Giang
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Communicated by M. H. Zenk

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Wysokińska, H., Giang, N.T.X. Selection of penstemide and serrulatoloside producing clones in penstemon serrulatus by small-aggregate cloning. Plant Cell Reports 9, 378–381 (1990). https://doi.org/10.1007/BF00232403

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  • DOI: https://doi.org/10.1007/BF00232403

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