Skip to main content
SpringerLink
Log in

Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure)

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of response for callus induction. Prolonged culture of these embryogenic calli on the original medium or subculture to an auxin-free medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. Chromosome counts from root-tip cells of anther-derived plant indicated that they were haploid (N=36).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

N6 :

Chu et al. (1975)

MS:

Murashige and Skoog (1962)

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

BA:

6-benzylaminopurine

References

  1. Alexander MP, Rao TC (1968) Curr. Sci. 37:415

    Google Scholar 

  2. Chu CC, Wang CC, Sun CS, Chan H, Yin KC, Chu CY, Bi FY (1975) Sci. Sinica 18:659–668.

    Google Scholar 

  3. Hassan AE, Debergh P (1987) Plant Cell Tissue Organ Culture 10:73–77.

    Google Scholar 

  4. Huang SC, Murashige T (1983) Bot. Bull. Acad. Sin. 24:31–52.

    Google Scholar 

  5. Mehta UI, Rao VR, Ram HYM (1982) In:Proc. 5th Int Congr Plant Tissue Cell Culture, pp 109–110.

  6. Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–479.

    Google Scholar 

  7. Nadgauda RS, Parasharami VA, Mascarenhas AF (1990) Nature 344:335–336.

    Google Scholar 

  8. Paka K, Widholm JM (1984) Plant Mol. Biol. Rep. 2:37–42.

    Google Scholar 

  9. Rao IO, Rao IVR, Narang V (1985) Plant Cell Rep. 4:191–194.

    Google Scholar 

  10. Tseng TC, Liu DF, Shaio SY (1975) Bot. Bull. Acad. Sin. 16:55–66.

    Google Scholar 

  11. Yen ML, Chang WC (1986a) Theor. Appl. Genet. 73:161–163.

    Google Scholar 

  12. Yeh ML, Cheng WC (1986b) Plant Cell Rep. 5: 409–411.

    Google Scholar 

  13. Yeh ML, Chang WC (1987) Plant Sci. 51:93–96.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy, Taiwan Agricultural Research Institute, Taichung, Taiwan 41301, Republic of China

    H. S. Tsay, C. C. Yeh & J. Y. Hsu

Authors
  1. H. S. Tsay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. C. Yeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Y. Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. M. Widholm

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsay, H.S., Yeh, C.C. & Hsu, J.Y. Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure). Plant Cell Reports 9, 349–351 (1990). https://doi.org/10.1007/BF00232396

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232396

Keywords

Search

Navigation