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Arabidopsis thaliana: protocol for plant regeneration from protoplasts

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Abstract

We report a protocol for plant regeneration from leaf protoplasts of Arabidopsis thaliana ecotype Zürich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in plant regeneration from protoplast-derived clones is highly variable. In the hands of one of us (H.K.) average shoot regeneration frequency (% of calli regenerating at least one shoot) was ca. 60% and average plant regeneration frequency (% of calli yielding fertile plants in soil) was ca. 40%.

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Abbreviations

2,4-D :

2,4-dichlorophenoxyacetic acid

IAA :

indoleacetic acid

NAA :

αnaphthaleneacetic acid

BAP :

benzylaminopurine

2-iP :

2-isopentenylaminopurine

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Authors and Affiliations

  1. Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), ETH-Zentrum, CH-8092, Zürich, Switzerland

    Hans Karesch, Roland Bilang & Ingo Potrykus

Authors
  1. Hans Karesch
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  2. Roland Bilang
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  3. Ingo Potrykus
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Communicated by H. Lörz

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Karesch, H., Bilang, R. & Potrykus, I. Arabidopsis thaliana: protocol for plant regeneration from protoplasts. Plant Cell Reports 9, 575–578 (1991). https://doi.org/10.1007/BF00232335

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  • DOI: https://doi.org/10.1007/BF00232335

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