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Plant regeneration from cotyledon protoplasts of Xinjiang muskmelon

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Abstract

Cotyledon protoplasts were isolated from seedlings of Xinjiang muskelon (Cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified Miller medium. High frequency division of the protoplast-derived cells was observed. Agarose bead culture with B6S3 tobacco crown gall nurse cells was found most suitable for the cotyledon protoplasts when compared with other culture methods. Intact plants were regenerated from the protocalli by a two-step culture procedure with liquid and then solid media.

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Abbreviations

BAP:

6-benzylaminopurine

B6S3 :

crown gall tumor cells of tobacco

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

MES:

2(Nmorpholino) ethanesulfonic acid

MS:

Murashige and Skoog medium(1962)

NAA:

α -naphthaleneacetic acid

N6:

Zhu et al. medium (1975)

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Authors and Affiliations

  1. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Wulumuqi, People's Republic of China

    Renjing Li

  2. Institute of Genetics, Academia Sinica, Beijing, People's Republic of China

    Yongru Sun, Liming Zhang & Xianghui Li

Authors
  1. Renjing Li
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  2. Yongru Sun
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  3. Liming Zhang
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  4. Xianghui Li
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Communicated by J. M. Widholm

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Li, R., Sun, Y., Zhang, L. et al. Plant regeneration from cotyledon protoplasts of Xinjiang muskmelon. Plant Cell Reports 9, 199–203 (1990). https://doi.org/10.1007/BF00232179

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  • DOI: https://doi.org/10.1007/BF00232179

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