Abstract
Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to direct embryo formation were investigated, and the nature of the callus used for protoplast preparation was found to be a critical factor. The osmotic concentration and the composition of the culture medium including the phytohormone combinations were also important.
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Attree SM, Bekkaoui F, Dunstan DI, Fowkel (1987) Plant Cell Rep 6: 480–483
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci Sin 18: 659–668
Jia JF, Shi JH, Wang YM, Zhang SY (1989) Acta Botanica Sin 31: 361–366
Kao KN, Michayluk MR (1975) Planta 126: 105–110
Li ZY, Chen HM (1986) Acta Botanica Sin 28: 50–54
Li ZY, Chen HM (1987) Acta Botanica Sin 29: 354–356
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Sheng SH, Chen HM (1990) Acta Botanica Sin 32: 268–273
Vasil V, Vasil IK (1980) Theor Appl Genet 56: 97–99
Wang JM, Chen HM (1991) Acta Botanica Sin 33: 261–266
Zhang SP, Jia JF, Li HR, Zhang GC (1985) Chin Sci Bull (Chinese ed.). 18: 1423–1425
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Communicated by I.K. Vasil
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Guang-min, X., Zhongyi, L., Guang-qin, G. et al. Direct somatic embryogenesis and plant regeneration from protoplasts of Bupleurum scorzonerifolium Willd. Plant Cell Reports 11, 155–158 (1992). https://doi.org/10.1007/BF00232170
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DOI: https://doi.org/10.1007/BF00232170