Summary
Shoot tip explants, callus and embryogenic cell suspensions of Euphorbia pulcherrima have been examined for quantitative variation in nuclear DNA content by means of cytophotometry. Increasing instability was found in calli and cell suspensions from Erlenmeyer flask and bioreactor culture. Nuclear DNA content ranged from 2C up to 32 C. Plants regenerated from embryogenic cell suspensions, however, were highly uniform with regard to phenotype and ploidy level indicating strongly impaired embryogenic potential of polyploid, aneuploid or other genetically altered cells.
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References
Ammirato PV (1989) Recent progress in somatic embryogenesis. IAPTC Newsletter 57:2–16
Armstrong CL, Phillips RL (1988) Genetic and cytogenetic variation in plants regenerated from organogenic and friable, embryogenic tissue cultures of maize. Crop Sci 28:363–368
Barwale UB, Widholm JM (1987) Somaclonal variation in plants regenerated from cultures of soybean. Plant Cell Rep 6:365–368
Bayliss MW (1977) The causes of competition between two cell lines of Daucus carota in mixed culture. Protoplasma 92:117–127
Binarová P, Dolezel J (1988) Alfalfa embryogenic cell suspension culture: Growth and ploidy level stability. J Plant Physiol 133:561–566
Cassells AC, Minas G (1983) Beneficially-infected and chimeral pelargoniums: implications for micropropagation by meristem and explant culture. Acta Horticulturae 131:287–297
Cavallini A, Lupi MC, Cremonini R, Bennici A (1987) In vitro culture of Bellevalia romana (L.) Rchb. III. Cytological study of somatic embryos. Protoplasma 139:66–70
D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. Crit Rev Plant Sci 3:73–112
Dole J, Wilkins H (1988) A graft-transmissible factor in Euphorbia pulcherrima causing permanent changes in branching and other morphological characteristics. Acta Horticulturae 226:283–291
Fish N, Karp A (1986) Improvements in regeneration from protoplasts of potato and studies on chromosome stability. Theor Appl Genet 72:405–412
Geier T (1991) Chromosome Variability in Callus Produced Plants. In: Harding J, Singh F, Mol JNM (eds) Genetics and Breeding of Ornamental Species. Kluwer Academic Publishers, pp 79–106
Karp A, Bright SWJ (1985) On the causes and origins of somaclonal variation. Oxford Surv Plant Molec Cell Biol 2:199–234
Kleffel B, Walther F, Preil W (1986) X-ray-induced mutability in embryogenic suspension cultures of Euphorbia pulcherrima. In: Nuclear Techniques and in vitro Culture for Plant Improvement IAEA Vienna, pp 113–120
Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Ann Rev Plant Physiol Plant Mol Biol 39:413–437
Lupi MC, Bennici A, Baroncelli S, Gennai D, D'Amato F (1981) In vitro regeneration of Durum wheat plants. II. Diplontic selection in aneusomatic plants. Z Pflanzenzüchtg 87:167–171
Meins F Jr (1983) Heritable variation in plant cell culture. Ann Rev Plant Physiol 34:327–346
Orton TJ (1987) Genetic instability in celery tissue and cell cultures. Iowa State J Res 61:481–498
Phillips RL, Kaeppler SM, Peschke VM (1990) Do we understand somaclonal variation? In: Nijkamp HJJ, van der Plas LHW, van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer Academic Publishers, pp 131–141
Preil W (1991) Application of bioreactors in plant propagation. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer Academic Publishers, pp 425–445
Preil W, Beck A (1991) Somatic embryogenesis in bioreactor culture. Acta Horticulturae 289:179–192
Preil W, Engelhardt M (1982) In vitro-Entmischung von Chimärenstrukturen durch Suspensionskulturen bei Euphorbia pulcherrima Willd. Gartenbauwiss 47:241–244
Rajasekaran K, Schank SC, Vasil IK (1986) Characterization of biomass production, cytology and phenotypes of plants regenerated from embryogenic callus cultures of Pennisetum americanum x P. purpureum (hybrid triploid napiergrass) Theor Appl Genet 73:4–10
Skene KGM, Barlass M (1983) Studies on the fragmented shoot apex of grapevine. IV. Separation of phenotypes in a periclinal chimera in vitro. J Exp Bot 34:1271–1280
Skirvin RM, Janick J (1976) Tissue culture-induced variation in scented Pelargonium spp.. J Amer Soc Hort Sci 101:281–290
Stimart DP (1983) Promotion and inhibition of branching in Poinsettia in grafts between self-branching and nonbranching cultivars. J Amer Soc Hort Sci 108:419–422
Swedlund B, Vasil IK (1985) Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum (L.) K.Schum. Theor Appl Genet 69:575–581
Vanzulli L, Magnien E, Olivi L (1980) Caryological stability of Datura innoxia calli analysed by cytophotometry for 22 hormonal combinations. Pl Sci Lett 17:181–192
Varga A, Thoma LH, Bruinsma J (1988) Effects of auxins and cytokinins on epigenetic instability of calluspropagated Kalanchoe blossfeldiana Poelln. Plant Cell Tiss Org Cult 15:223–231
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Geier, T., Beck, A. & Preil, W. High Uniformity of plants regenerated from cytogenetically variable embryogenic suspension cultures of poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch). Plant Cell Reports 11, 150–154 (1992). https://doi.org/10.1007/BF00232169
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DOI: https://doi.org/10.1007/BF00232169