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Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance

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Summary

A modified root explant transformation method has been developed that is effective in producing transgenic Arabidopsis thaliana plants which are methotrexate resistant due to the integration of T-DNA vectors containing a chimeric dihydrofolate reductase gene. Molecular analysis shows that transformed methotrexate resistant plants contain the expected T-DNA construct with the chimeric gene. This transformation method also works well with other plant selectable markers, including hygromycin phosphotransferase and neomycin phosphotransferase II.

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Abbreviations

DHFR:

(dihydrofolate reductase)

HPT:

(hygromycin phosphotransferase)

NPTII:

(neomycin phosphotransferase)

CaMV:

(Cauliflower Mosaic Virus)

MES:

(2-[N-morpholino]ethanesulfonic acid)

BAP:

(N6-benzylaminopurine)

NAA:

(naphthaleneacetic acid)

2,4-D:

(2,4 dichlorophenoxyacetic acid)

2iP:

(6-(dimethylallylamino)-purine)

2iPAde:

(6-(dimethylallylamino)-ade-nine)

IAA:

(indole-3-acetic acid)

IBA:

(indole-3-butyric acid)

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany

    Elke Kemper, Christoph Grevelding, Jeff Schell & Robert Masterson

Authors
  1. Elke Kemper
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  2. Christoph Grevelding
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  3. Jeff Schell
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  4. Robert Masterson
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Additional information

Communicated by H. Lörz

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Kemper, E., Grevelding, C., Schell, J. et al. Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance. Plant Cell Reports 11, 118–121 (1992). https://doi.org/10.1007/BF00232162

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  • DOI: https://doi.org/10.1007/BF00232162

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