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Expression of two cytochromes P450 involved in carcinogen activation in a human colon cell line

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Abstract

Cytochrome P450 is known to cause carcinogen activation and correspondingly increased cancer risk in animal models. In order to determine whether P450 in the colon may be involved in cancer development in the human, the human colon cell line LS174T was examined for the presence of various cytochromes P450. Two isozymes of P450 were identified in the human cell line. Expression of P450IAl or IA2 was increased by treatment of the cell line with benzanthracene; the induction was demonstrated by an increase in RNA hybridizing to a probe for P4501Al and by ethoxyresorufin deethylation activity. Western analysis of microsomes isolated from human colon tissue also demonstrated the presence of P4501A1, as well as a form which cross-reacted to an antibody to human P450IIC9. Another isozyme, P450IIE1, was identified by polymerase chain reaction amplification of RNA from LS174T cells. These results underscore the presence of cytochromes P450 in colonic tissue and provide a basis for the involvement of isozyme-specific P450 mediated reactions in carcinogenesis of the colon.

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Author notes

  1. Hernán Vásquez

    Present address: Department of Biochemistry, Faculty of Medicine, Universidad de Chile, Santiago-7, Chile

Authors and Affiliations

  1. The Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, P.O. Box 20708, 77225, Houston, Texas, USA

    Terry B. White, Dianne K. Hammond, Hernán Vásquez & Henry W. Strobel

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  1. Terry B. White
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  2. Dianne K. Hammond
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  3. Hernán Vásquez
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  4. Henry W. Strobel
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Additional information

Some of the data presented here were taken from a thesis submitted by D.K.H. in partial fulfillment of the requirements for the Ph.D. degree in the University of Texas Graduate School of Biomedical Sciences.

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White, T.B., Hammond, D.K., Vásquez, H. et al. Expression of two cytochromes P450 involved in carcinogen activation in a human colon cell line. Mol Cell Biochem 102, 61–69 (1991). https://doi.org/10.1007/BF00232158

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  • DOI: https://doi.org/10.1007/BF00232158

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