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Plant Cell Reports

, Volume 9, Issue 1, pp 1–5 | Cite as

Intrinsic GUS-like activities in seed plants

  • Ching-yeh Hu
  • Paula P. Chee
  • Robert H. Chesney
  • James H. Zhou
  • Paul D. Miller
  • W. Timothy O'Brien
Article

Abstract

Fifty-two plant species, covering some Gymnosperms and all the key groups of Angiosperms, were chosen for surveying their intrinsic beta-glucuronidase-like activities. Histochemical (overnight incubation) and qualitative fluorometric (24 h incubation) assays indicated that, with few exceptions, such activities were detected in certain part(s) of the fruit walls, seed coats, endosperms or, especially, the embryos of the tested plants. Most of such activities in the excised immature embryos of soybean and string bean disappeared after one to a few days' in vitro culturing. Such activities in the intact mature seeds of these two species diminished also during germination process. The vegetative organs of seedlings/mature plants usually lack such activities. The enzyme(s) responsible for such activities was antigenically dissimilar to E. coli beta-glucuronidase.

Keywords

Plant Species Seed Coat Overnight Incubation Seed Plant Mature Seed 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

B5

basal medium described by Gamborg et al. (1968)

GUS

beta- glucuronidase

MUG

4-methyl umbelliferyl glucuronide

X-Glu

5-bromo-4-chloro-3-indolyl glucuronide

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ching-yeh Hu
    • 1
  • Paula P. Chee
    • 2
  • Robert H. Chesney
    • 1
  • James H. Zhou
    • 3
  • Paul D. Miller
    • 4
  • W. Timothy O'Brien
    • 1
  1. 1.Biology DepartmentWilliam Paterson CollegeWayneUSA
  2. 2.Molecular Biology Division, The Upjohn Co.KalamazooUSA
  3. 3.Department of GeneticsIowa State UniversityAmesUSA
  4. 4.DNA Plant Technology Corp.CinnaminsonUSA

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