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Plant regeneration from callus cultures of Valeriana wallichii DC.

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Abstract

Petiole expiants of Valeriana wallichii. DC., a threatened medicinal plant, were used for inducing callus. Optimum callus formation was observed on Murashige and Skoogs' (1962) medium supplemented with 3.0 mg/l NAA and 0.25 mg/l Kn. Shoot regeneration was achieved upon transferring the callus to medium containing 1.0 mg/l Kn and 0.25 mg/l NAA. Complete plantlets were obtained on the same medium or upon transfer of the regenerated shoot buds to medium containing 5.0 mg/l Kn and 1.0 mg/l IAA. Nearly a thousand callus regenerated plants were successfully transferred to the field following previously standardized hardening procedures.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-dichloro phenoxyaceticacid

2iP:

2-isopentenyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

Kinetin

MS:

Murashige and Skoog's medium (1962)

NAA:

α-napthalene aceticacid

Z:

Zeatin

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, University of Gorakhpur, 273 009, Gorakhpur, India

    Jaideep Mathur

  2. Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants, P.O. Box No. 1, 226 016, RSM Nagar, Lucknow, India

    Paramvir Singh Ahuja

Authors
  1. Jaideep Mathur
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  2. Paramvir Singh Ahuja
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Additional information

Communicated by J.M. Widholm

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Mathur, J., Singh Ahuja, P. Plant regeneration from callus cultures of Valeriana wallichii DC.. Plant Cell Reports 9, 523–526 (1991). https://doi.org/10.1007/BF00232111

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  • DOI: https://doi.org/10.1007/BF00232111

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