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Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum)

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Abstract

Protoplasts have been isolated from three tuber-bearing Solanum species, S. hjertingii, S. polyadenium and S. capsicibaccatum, that are sexually incompatible with S. tuberosum, but possess potentially useful characters. For isolating protoplasts from leaves of in vitro shoot cultures of S. hjertingii and S. capsicibaccatum growth was improved by including silver thiosulfate in the medium. However, for S. polyadenium, leaves of pot-grown plants were the best source for protoplasts. Following protoplast division and culture, plants were regenerated from protoplasts of each of the species. The pattern of chromosome variation in regenerants was similar to that observed for other diploid and tetraploid Solanum species. The results indicate that it should be possible to introduce the potentially useful germplasm from these wild species into somatic hybrids with S. tuberosum by protoplast fusion.

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Abbreviations

STS:

silver thiosulfate

BAP:

benzylaminopurine

GA3 :

gibberellic acid

NAA:

naphthalene acetic acid

IAA:

indole-3-acetic acid

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Author notes

  1. Y. -S. Xu & E. Pehu

    Present address: Department of Crop Husbandry, University of Helsinki, Helsinki, Finland

  2. M. G. K. Jones

    Present address: Plant Sciences, School of Biological and Environmental Sciences, Murdoch University, 6150, Perth, W. Australia

Authors and Affiliations

  1. Institute of Arable Crops Research, Rothamsted Experimental Station, 5 2JQ, Harpenden, Herts AL, UK

    Y. -S. Xu, E. Pehu, R. Malone & M. G. K. Jones

Authors
  1. Y. -S. Xu
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  2. E. Pehu
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  3. R. Malone
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  4. M. G. K. Jones
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Communicated by H. Lörz

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Xu, Y.S., Pehu, E., Malone, R. et al. Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum). Plant Cell Reports 9, 520–522 (1991). https://doi.org/10.1007/BF00232110

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  • DOI: https://doi.org/10.1007/BF00232110

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