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Verbascoside production by plant cell cultures

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Abstract

Verbascoside was found to be produced in all calli derived from eleven species that contained the compound in their leaves. Cell suspension cultures were also established in three species, i.e., Leucosceptrum japonicum f. barbinerve, Syringa josikaea, and Sy. vulgaris, all of which were found to produce verbascoside at more than 1 g/l. Of the three species, suspension cultures of L. japonicum f. barbinerve showed rapid growth and the highest yield of verbascoside (1.89 g/l). In these cultures, the effects of major salt concentration in B5 medium on cell growth and verbascoside production were examined. Maximum cell growth and maximum verbascoside production were both achieved by reducing the major salt concentration to half that of the original medium.

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Authors and Affiliations

  1. Research Institute for Biology and Chemistry, Tsumura and Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, 300-11, Ibaraki, Japan

    Nobuyuki Inagaki, Hiroaki Nishimura, Minoru Okada & Hiroshi Mitsuhashi

Authors
  1. Nobuyuki Inagaki
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  2. Hiroaki Nishimura
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  3. Minoru Okada
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  4. Hiroshi Mitsuhashi
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Communicated by M. Tabata

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Inagaki, N., Nishimura, H., Okada, M. et al. Verbascoside production by plant cell cultures. Plant Cell Reports 9, 484–487 (1991). https://doi.org/10.1007/BF00232101

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  • DOI: https://doi.org/10.1007/BF00232101

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