Skip to main content
SpringerLink
Log in

Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33°C one day heat treatment was generally necessary to induce embryogenesis. Analysis of ploidy level through flow cytometry for two cultivars indicated that haploids were present.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

DAPI:

4′,6′-diamidino-2-phenylindol

NAA:

naphthalene acetic acid

DH:

doubled haploid

References

  • Baillie AMR, Epp DJ, Hutcheson DS, Keller WA (1992) Plant Cell Reports 11: 234–237

    Google Scholar 

  • Beversdorf WD, Charne DG, Kott LS, Chuong PV, Polsoni L, Zilka J (1987) 7th Intern. Rapeseed Congress, Poznan 1: 86–91

    Google Scholar 

  • Burnett L, Yarrow S, Huang B (1992) Plant Cell Reports 11: 215–218

    Google Scholar 

  • Cao MQ, Charlot F, Doré C (1990) C.R. Acad Sci. Paris 310 Série III: 203–209

    Google Scholar 

  • Chuong PV, Beversdorf WD (1985) Plant Sci. 39: 219–226

    Google Scholar 

  • Detchepare S, Herzmann P, Dumas C (1989) J. Plant Physiol. 135: 129–137

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158

    Google Scholar 

  • Hetz E, Schieder O (1989) Abstract In: Science for Plant Breeding, 12th Eucarpia Congress, Göttingen, 25–10

  • Li CW (1984) In: Chinese cabbage in China, Agricultural Publishing House, Beijing, pp 1–27

    Google Scholar 

  • Lichter R (1982) Z. Pflanzenphysiol. 105: 427–434

    Google Scholar 

  • Pechan PM (1989) Plant Cell Reports 8: 387–390

    Google Scholar 

  • Sato T, Nishio T, Hirai M (1989) Plant Cell Reports 8: 486–488

    Google Scholar 

  • Thiagarajah MR, Stringam GR (1990) Abstract In: 7th Int. Congr. Pl. Tiss. Cell Cult., Amsterdam, p.190

  • Vergne P, Delvallée I, Dumas C (1987) Stain Technology 62: 299–304

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Beijing Vegetable Research Centre, P.O. Box 2443, 100081, Beijing, China

    Ming Qing Cao, Yan Li & Fan Liu

  2. INRA Station de Génétique et d'Amélioration des Plantes, Rte de Saint-Cyr, F-78026, Versailles Cedex, France

    Claire Doré

Authors
  1. Ming Qing Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Claire Doré
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Pelletier

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cao, M.Q., Li, Y., Liu, F. et al. Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture. Plant Cell Reports 13, 447–450 (1994). https://doi.org/10.1007/BF00231964

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00231964

Keywords

Search

Navigation