Skip to main content
Log in

Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Isolated microspore culture techniques are being widely used in Brassica breeding programs to generate haploid and doubled haploid plants. A number of factors influence regeneration response in vitro including genotype. In order to assess the effect of genotype on microspore embryogenesis in B. rapa L. var. oleifera, 17 cultivars and breeding lines were evaluated. Embryos developed from all but one genotype when using NLN medium with 17% sucrose, followed by a reduction in sucrose concentration to 10%, 48 h later. The number of embryos /100 buds differed between genotypes, ranging from 0 to 70. Further studies indicated that sucrose concentration and incubation time influenced embryogenesis. Selection studies carried out with an Agriculture and Agri-Food Canada breeding line have resulted in the identification of a highly embryogenic B. rapa line. This line produced thousands of microspore-derived embryos /100 buds and will be useful in mutant selection and gene transfer as well as biochemical and developmental studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arnison PG, Keller WA (1990) Plant Breeding 104:125–133.

    Google Scholar 

  • Baillie AMR, Epp DJ, Keller WA, Hutcheson D (1992) Plant Cell Rep. 11:234–237

    Google Scholar 

  • Burnett L, Yarrow S, Huang B (1992) Plant Cell Rep. 11:215–218.

    Google Scholar 

  • Cho MS, Zapata FJ (1988) Plant Sci. 58:239–244.

    Google Scholar 

  • Coumans MP, Sohata S, Swanson EB (1989) Plant Cell Rep. 7:618–622.

    Google Scholar 

  • Dunwell JM, Cornish M, Decourcel AGL, Middlefell-Williams JE (1985) J. Exp. Bot 36:1768–1778.

    Google Scholar 

  • Dunwell JM, Thurling N (1985) J. Exp. Bot. 36:1478–1491.

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158.

    Google Scholar 

  • Govil S, Babbar SB, Gupta SC (1986) Plant Breed. 97:64–71.

    Google Scholar 

  • Keller WA, Arnison PG, Cardy BJ (1987) Plant Tissue and Cell Culture, Green CE, Somers DA, Hackett WP, Giesboer DD (eds) Alan Liss Inc., NY, 223–241.

    Google Scholar 

  • Lichter R (1982) Z. Pflanzenphysiol. 105:427–434.

    Google Scholar 

  • Roulund N, Andersen SB, Farestveit B (1991) Euphytica 52:125–129.

    Google Scholar 

  • Swanson EB, Coumans MP, Brown GL, Patel JD, Beversdorf WD (1988) Plant Cell Rep 7:83–87.

    Google Scholar 

  • Swanson EB, Erickson LR (1989) Theor.Appl.Genet. 78:831–835.

    Google Scholar 

  • Taylor DC, Weber N, Underhill EW, Pomeroy MK, Keller WA, Scowcroft WR, Wilen RW, Moloney MM, Holbrook LA (1990) Planta 181:18–26.

    Google Scholar 

  • Taylor DC, Ferrie AMR, Keller WA, Giblin EM, Pass EW, MacKenzie SL (1993) Plant Cell Rep. 12:375–384.

    Google Scholar 

  • Ulrich A, Furton WH, Downey RK (1984) Sci. Counc. Can. Rep.

  • Wei ZM, Harada H (1986) Theor. Appl. Genet. 72:252–255.

    Google Scholar 

  • Yang Q, Chauvin JE, Herve Y (1992) Plant Cell, Tissue and Organ Culture 28:289–296.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by B.E. Ellis

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferrie, A.M.R., Epp, D.J. & Keller, W.A. Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line. Plant Cell Reports 14, 580–584 (1995). https://doi.org/10.1007/BF00231942

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00231942

Key words

Navigation