Abstract
Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
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Communicated by A. Komamine
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Fujimura, T., Kawai, T., Kajiwara, T. et al. Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales). Plant Cell Reports 14, 571–574 (1995). https://doi.org/10.1007/BF00231940
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DOI: https://doi.org/10.1007/BF00231940