Abstract
Transgenic rice plants (Oryza sativa cv. Nipponbare) carrying 1 or 2 copies of a rice homeobox gene, OSH1, under the control of the CaMV 35S promoter were generated. The transgene caused altered morphology of leaf, such as ligule-replacement and abnormal division of sclerenchyma cells. The phenotype of these leaves resembles that of maize leaf morphological mutant, Knotted 1, which is caused by duplication of the KN1 gene (Veit et al., 1990). The in situ hybridization analysis has revealed that the expression of endogenous OSH1 is mainly localized in developing vascular strands of stem. We have discussed the biological roles of OSH1 in rice based on these results.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Affolter, M., Schier, A. and Gehring, W.J. (1990) Current Opin. Cell Biol. 2, 485–495.
Akagi, H., Sakamoto, M., Negishi, T., and Fujimura, T. (1989) Mol. Gen. Genet. 215, 501–506.
Bellmann, R. and Werr, W. (1992) EMBO J. 11, 3367–3374.
Carabelli, M., Sessa, G., Baima, S., Morelli, G. and Ruberti, I. (1993) Plant J. 4, 469–479.
Freeling, M. and Hake, S. (1985) Genetics 111, 617–634.
Fujimura, T., Sakurai, M., Akagi, H., Negishi, T. and Hirose, A. (1985) Plant Tiss. Cult. Lett. 2, 74–75.
Hayashi, S. and Scott, M. P. (1990) Cell 63, 883–894.
Hoshikawa, K. (1989) The Growing Rice Plant, Nobunkyo Press, Tokyo, Japan.
Jackson, D., Veit, B. and Hake, S. (1994) Development 120, 405–413.
Kano-Murakami, Y., Yanai, T., Tagiri, A. and Matsuoka, M. (1993) FEBS Lett. 334, 365–368.
Kouchi, H. and Hata, S. (1993) Mol. Gen. Genet. 238, 106–119.
Mattsson, J., Söderman, E., Svenson, M., Borkird, C. and Engström, P. (1992) Plant Mol. Biol. 18, 1019–1022.
Matsuoka, M., Ichikawa, H., Saito, A., Tada, Y., Fujimura, T. and Kano-Murakami, Y. (1993) Plant Cell 5, 1039–1048.
McGinnis, W., Levin, M.S., Hafen, E., Kuroiwa, A. and Gehring, W. J. (1984) Nature 308, 428–433.
Ruberti, L., Sessa, G., Lucchetti, S. and Morelli, G. (1991) EMBO J. 10, 1787–1791.
Schena, M. and Davis, R. W. (1992) Proc. Natl. Acad. Sci. USA 89, 3894–3898.
Schena, M., Lloyd, A. M. and Davis, R. W. (1993) Genes Develop. 7, 367–379.
Schindler, U., Beckmann, H. and Cashmore, A. R. (1993) Plant J. 4, 137–150.
Scott, M. P. and Weiner, A. J. (1984) Proc. Natl. Acad. Sci. UAS 81, 4115–4119.
Scott, M. P., Tamkun, J. W., and Hartzell, G. W. III (1989) BBA Rev. Cancer 989, 25–48.
Sinha, N., Williams, R. and Hake, S. (1993) Genes Develop. 7, 787–795.
Smith, L. G., Greene, B., Veit, B. and Hake, S. (1992) Development 116, 21–30.
Tada, Y., Sakamoto, M., and Fujimura, T. (1990). Theor. Appl. Genet. 80, 1803–1808.
Veit, B., Vollbrecht, E., Mathern, J. and Hake, S. (1990) Genetics 125, 623–631.
Vollbrecht, E., Veit, B., Sinha, N. and Hake, S. (1991) Nature 350, 241–243.
Author information
Authors and Affiliations
Additional information
Communicated by K. Shimamoto
Rights and permissions
About this article
Cite this article
Matsuoka, M., Tamaoki, M., Tada, Y. et al. Expression of rice OSH1 gene is localized in developing vascular strands and its ectopic expression in transgenic rice causes altered morphology of leaf. Plant Cell Reports 14, 555–559 (1995). https://doi.org/10.1007/BF00231937
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00231937