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Forskolin synthesis in in vitro cultures of Coleus forskohlii Briq transformed with Agrobacterium tumefaciens

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Abstract

Agrobacterium tumefaciens mediated tumor tissue and shooty teratomas of Coleus forskohlii were cultured in vitro. Forskolin was detected in tumorous callus (0.002%), rhizogenic callus (0.011%) and root cultures (0.014%), but not in shooty teratomas. Forskolin synthesis and accumulation in tumorous C. forskohlii cultures may permit the elucidation of diterpene metabolism in this species.

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Abbreviations

B5 :

medium after Gamborg et al. (1968)

2,4-D:

2,4, dichlorophenoxyaceticacid

MS:

medium after Murashige and Skoog (1962)

BAP:

benzylaminopurine

IBA:

indole-3-butyric acid

Kn:

kinetin

CH:

casein hydrolysate

T-DNA:

transferred DNA

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Authors and Affiliations

  1. Centre of Advanced Study, Dept. Botany, Calcutta University, 35, Ballygunge Circular Road, 700019, Calcutta, India

    Swapna Mukherjee, Biswajit Ghosh & Sumita Jha

Authors
  1. Swapna Mukherjee
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  2. Biswajit Ghosh
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  3. Sumita Jha
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Communicated by F. Constabel

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Mukherjee, S., Ghosh, B. & Jha, S. Forskolin synthesis in in vitro cultures of Coleus forskohlii Briq transformed with Agrobacterium tumefaciens . Plant Cell Reports 15, 691–694 (1996). https://doi.org/10.1007/BF00231926

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  • DOI: https://doi.org/10.1007/BF00231926

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