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Differential expression of multiple forms of arginase in cultured cells

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Summary

Arginase (EC 3.5.3.1), the final enzyme in the urea cycle, catalyzes the cleavage of arginine to orthinine and urea. At least two forms of this enzyme, Al and All, have been described and are probably encoded by discrete genetic loci. The expression of these separate genes has been studied in mammalian cells grown in culture. The permanent rat-hepatoma line H4-II-E-C3 contained exclusively the Al enzyme; the form in mammals comprising about 98% of the arginase activity in liver and erythrocytes but catalyzing only about one half of that reaction in kidney, gastrointestinal tract, and brain. By contrast, human-embryonic-kidney and -brain cells, after transformation with the human papovavirus BK, contained only the All species of arginase, which form contributes the remaining half of that catalysis in those mammalian tissues in vivo. We report here the results of an extensive study on the properties of these two forms of arginase in the three cell lines, including Km values for arginine, behavior on polyacrylamide gels under non-denaturing conditions, and cross-reactivity with lapine antibodies against the arginases from either rat or human liver.[/p]

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Authors and Affiliations

  1. Departments of Pediatrics and Psychiatry and the Mental Retardation Research Center, University of California, Los Angeles, School of Medicine, 90024, Los Angeles, CA, USA

    Elaine B. Spector, Rita M. Kern, Donald F. Haggerty & Stephen D. Cederbaum

Authors
  1. Elaine B. Spector
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  2. Rita M. Kern
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  3. Donald F. Haggerty
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  4. Stephen D. Cederbaum
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Presented in part at the annual meeting of the Society for Pediatric Research, Washington, D.C., May, 1982. Pediatr. Res. 16:195A.

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Spector, E.B., Kern, R.M., Haggerty, D.F. et al. Differential expression of multiple forms of arginase in cultured cells. Mol Cell Biochem 66, 45–53 (1985). https://doi.org/10.1007/BF00231822

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