Summary
A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yield of protoplasts was released from suspension cells (2 to 3×107 protoplasts per ml packed cell volume) when treated with enzyme mixtures. The isolated protoplasts divided at a relatively high frequency (20% to 50%) in both liquid and agarose-solidified KM8p medium. Up to 0.21% of the dividing protoplasts continued to divide and form micro-calli. Sixty-eight plants were regenerated from micro-calli, and among the 30 plants which were transplanted to the greenhouse, 3 have survived.
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Abbreviations
- BAP:
-
6, enzylaminopurine
- 2,4-D:
-
2,4 dichlorophenoxyacetic acid
- DMSO:
-
dimethylsulfoxide
- FDA:
-
Fluorescein diacetate
- IAA:
-
indole-3-acetic acid
- LS:
-
Linsmaier and Skoog basal medium (1965)
- MES:
-
2, [N-morpholino]-ethanesulfonic acid
- MS:
-
Murashige and Skoog basal medium (1962)
- NAA:
-
1, naphthaleneacetic acid
- PCV:
-
packed cell volume
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Communicated by G. C. Phillips
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Chang, YF., Wang, W.C., Warfield, C.Y. et al. Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.). Plant Cell Reports 9, 611–614 (1991). https://doi.org/10.1007/BF00231799
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DOI: https://doi.org/10.1007/BF00231799