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Disassembly and reconstitution of yeast 60S ribosomal subunits

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Summary

Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 μmol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.

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Abbreviations

DMMA:

Dimethylmaleic Anhydride

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Authors and Affiliations

  1. Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049, Canto Blanco, Madrid, Spain

    Maria Angela Nieto, Francisco Hernández & Enrique Palacián

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  1. Maria Angela Nieto
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  2. Francisco Hernández
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  3. Enrique Palacián
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Nieto, M.A., Hernández, F. & Palacián, E. Disassembly and reconstitution of yeast 60S ribosomal subunits. Mol Cell Biochem 86, 55–63 (1989). https://doi.org/10.1007/BF00231689

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  • DOI: https://doi.org/10.1007/BF00231689

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