Abstract
The role of ethylene in microspore embryogenesis and regeneration was analyzed by studying the effects of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and the ethylene antagonists silver nitrate and silver thiosulphate on the androgenic response of in vitro cultured anthers of seven genotypes of barley. Incorporation of either ACC or silver salts in the culture medium lead to a significant increase in callus induction for five of the seven genotypes tested. The treatment that increased callus induction depended upon genotype. Only anthers cultured on 1 mg l−1 silver thiosulphate gave rise to fertile plants in all seven genotypes tested.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole acetic acid
- PAA:
-
phenyl acetic acid
- STS:
-
silver thiosulphate
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
References
Babbar SB, Gupta C (1986) Physiol Plant 68:141–144
Biddington NL (1992) Plant Growth Regulation 11:173–187
Cho UH, Kasha K (1989) Plant Cell Reports 8:415–417
Cho UH, Kasha KJ (1992) J Plant Physiol 139:299–302
De Block M (1988) Theor Appl Genet 76:767–774
Komatsuda T, Enomoto S, Nakajima K (1989) Journal of Heredity 80:345–350
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Olsen FL (1987) Carlsberg Res Comm 52:393–404
Palmer CE (1992) Plant Cell Reports 11:541–545
Perl A, Aviv D, Galun E (1988) Plant Cell Reports 7:403–406
Pius J, George L, Eapen S, Rao PS (1993) Plant Cell Tissue and Organ Culture 32:91–96
Powell W (1987) Genome 30:152–157
Purnhauser L, Medgyesy P, Czako M, Dix PJ, Marton L (1987) Plant Cell Reports 6:1–4
Radke SE, Turner JC, Facciotti D (1992) Plant Cell Reports 11:499–505
Salisbury FB, Ross CW (1992) Plant Physiology, 4th ed, Wadsworth Publishing Company, Belmont, CA: 393–395
Sethi U, Basu A, Guha-Mukherjee S (1990) Plant Sci 69:225–229
Songstad DD, Armstrong CL, Petersen WL (1991) Plant Cell Reports 9:699–702
Song HG, Li SN, Li GR, Yun SG, Li JW (1981) In: Plant Tissue Culture, Proceeding of the Beijing (Peking) Symposium. Pitman Publ Ltd. London: pp 97–106
Tiainen T (1992) Plant Cell Reports 10: 604–607
Vain P, Flament P, Soudain P (1989) J.Plant Physiol 135:537–540
Wang C-C, Sun C-S, Chu Z-C (1974) Acta Bot Sin 16:43–54
Yang SF, Huffman NE (1985) Ann Rev of Plant Physiol 35:155–189
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Communicated by M. R. Davey
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Evans, J.M., Batty, N.P. Ethylene precursors and antagonists increase embryogenesis of Hordeum vulgare L. anther culture. Plant Cell Reports 13, 676–678 (1994). https://doi.org/10.1007/BF00231622
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DOI: https://doi.org/10.1007/BF00231622