Abstract
The potato (Solanum tuberosum) genome contains a highly heterogeneous population of Ty1-copia group retrotransposons. Here we identify the first such transposable element known to be transcribed in this species. The elements are transcriptionally activated during protoplast isolation. The majority of the activated Ty1-copia sequences are similar to elements which are transcriptionally induced under the same conditions in tobacco (Nicotiana tabacum). We also show that a previously identified potato element M166, which has no known equivalent in tobacco is also transcribed under these conditions. It appears that the control of transcription of this particular Ty1-copia group retrotransposons has been broadly conserved between these two species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- LTR:
-
long terminal repeats
- PCR:
-
polymerase chain reaction
References
Arnault C, Deformel I (1994) Genetica 93:149–160
Casacuberta JM, Grandbastien M-A (1993) Nucl Acid Res 21:2087–2093
Casacuberta JM, Vernhettes S, Grandbastien M-A (1995) EMBO J 14:2670–2678
Cooper-Bland S, De, Maine MJ, Fleming MLMH, Phillips MS, Powell W, Kumar A (1994) J Exp Bot 45:1319–1326
Doolittle RF, Feng D (1990) In: Doolittle RF (ed) Methods in Enzymology, Vol 183, Academic Press, New York, pp 659–669
Feng D, Doolittle RF (1990) In: Doolittle RF (ed) Methods in Enzymology, Vol 183, Academic Press, New York, pp 375–387
Flavell AJ, Smith D, Kumar A (1992a) Mol Gen Gen 231:233–242
Flavell AJ, Dumbar E, Anderson R, Pearce SR, Hartley R, Kumar A (1992b) Nucl Acid Res 20:3639–3644
Flavell AJ, Pearce SR, Kumar A (1994) Current Opinion Genet Dev 4:838–844
Grandbastien M-A, Spielmann A, Caboche M (1989) Nature 337:376–380
Grandbastien M-A, Audeoun C, Casacuberta JM, Grappin P, Lucas H, Moreau C, Pouteau S (1994) Genetica 93:181–189
Higgins DG, Sharp PM (1988) Gene 73:237–244
Hirochika H (1993) EMBO J 12:2521–2528
Hirochika H, Fukuchi A, Kikuchi F (1992) Mol Gen Genet 233:209–216
Hirochika H, Hirochika R (1993) Jpn J Genet 68:35–46
Konieczny A, Voytas DF, Cummings MP, Ausubel FM (1991) Genetics 127:801–809
Kumar A (1996) Trends in Genet 12:41–43
McClintock B (1984) Science 226:792–801
Pauls PK, Kunert K, Huttner E, Grandbastien M-A (1994) Plant Mol Biol 26:393–402
Pearce SR, Harrison G, Li D, Heslop-Harrison JS, Kumar A, Flavell A (1995) Mol Gen Genet 250:305–315
Pouteau S, Huttner E, Grandbastien M-A, Caboche M (1991) EMBO J 10:1911–1918
Pouteau S, Grandbastien M-A, Boccara M (1994) Plant J 5:535–542
Ricci P, Panabieres F, Bonnet P, Maia M, Ponchet M, Devergne JC, Marais A, Cardin L, Milat ML, Blein JP (1993) In: Fritig B, Legrand M (ed) Mechanisms of Plant Defence Responses, Kluwer Academic Publishers NL, pp 121–135
Sambrook J, Fritsh FF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, New York
Voytas DF, Cummings MP, Konieczny A, Ausubel FM, Rodermels (1992) Proc Natl Acad Sci USA 89:7124–7128
WanderWiel PL, Voytas D, Wendel JF (1993) J Mol Evol 36:429–447
Author information
Authors and Affiliations
Additional information
Communicated by I. Potrykus
Rights and permissions
About this article
Cite this article
Pearce, S.R., Kumar, A. & Flavell, A.J. Activation of the Ty1-copia group retrotransposons of potato (Solatium tuberosum) during protoplast isolation. Plant Cell Reports 15, 949–953 (1996). https://doi.org/10.1007/BF00231594
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00231594