Summary
When interested in plant cell transformation, the cell wall is often considered as a barrier to DNA transfer, which is only overcome by wounding or wall degrading enzymes. In this work, we demonstrate that cell plasmolysis before electropulsation is an efficient approach to DNA delivery into intact plant cells. Using such a method, transient expression (β-glucuronidase and chloramphenicol acetyltransferase) and stable expression (phosphinotricin acetyltransferase) of exogenous genes are obtained in intact black Mexican sweet maize cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BMS cells:
-
Black Mexican Sweet cells
- GUS:
-
β-glucuronidase
- CAT:
-
chloramphenicol acetyltransferase
- PAT:
-
Phosphinotricin acetyltransferase
- MS:
-
Murashige and Skoog
- PCV:
-
packed cell volume
- 4-MU and 4-MUG:
-
4-methylumbelliferone and 4-methylumbelliferyl-glucuronide
- BSA:
-
bovine serum albumin
- TTC:
-
triphenyl tetrazolium chloride
References
Arencibia A, Molina PR, de la Riva G, Guillermo SH (1995) Plant Cell Reports 14: 305–309
Aves K, Genovesi D, Willets N, Zachweija S, Mann M, Spencer T, Hick C, Gordon-Kamm W (1992) In Vitro 28: 124A
Baron-Epel O, Gharial PK, Schindler M (1988) Planta 175: 389–395
Carpita N, Sabularse D, Montezinos D, Delmer DP (1979) Science 205: 1144–1147
Dekeyser RD, Claes B, De Rycke RMU, Habets ME, Van Montagu MC, Caplan AB (1990) Plant Cell 2: 591–602
D'Hallium K, Bonne E, Bossut M, De Beuckeleer M, Leemans J (1992) Plant Cell 4: 1495–1505
Dixon RA (1985) Plant cell culture. A practical approach, Edited by RA. Dixon pp: 1–20
Eynard N, Sixou S, Duran N, Teissié J (1992) Eur J Biochem 209: 431–436
Fleck J, Durr A, Lett MC, Hirth L (1979) Planta 145: 279–285
Fromm ME, Taylor LP, Walbot V (1986) Nature 319: 791–793
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Bio/Technol. 8: 833–839
Gordon-Kamm WJ, Spenser TM, Mangano R, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Plant Cell 2: 603–618
Ingelbrecht ILW, Herman LMF, Dekeyzer RA, Van Montagu MC, Depicker AG (1989) Plant Cell 1: 671–680
Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405
Koziel MG, Beland GL, Bowman C, Carozzi NB, Crenshaw R, Launis K, Lewis K, Maddox D, McPherson K, Meghji MR, Merlin E, Rhodes R, Wright M, Evola SV (1993) Bio/Technol 11: 194–200
Loveys BR, Robinson SP (1987) Plant Science 49: 23–30
Laursen CM, Krzyzek RA, Flick CF, Anderson PC, Spenser TM (1994) Plant Mol Biol 24: 51–61
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Pescitelli SM, Sukhapinda K (1995) Plant Cell Reports 14: 712–716
Potrykus I (1990) Bio/Technol 8: 535–542
Rhodes CA, Pierce DA, Meuler IJ, Mascarenhas D, Detmer JJ (1988) Science 240: 204–207
Spenser TM, Gordon-Kamm WJ, Daines RJ, Start WG, Lemaux PG (1990) Theor Appl Genet 79: 625–631
Songstad DD, Halaka FG, DeBoer DL, Armstrong CL, Hinchee MAW (1993) Plant Cell Tissue Organ Culture 33: 195–201
Tada Y, Sakamoto M, Fujimura T (1990) Theor Appl Genet 80: 475–480
Vemet J, Fleck J, Durr A, Fritsch C, Pinck M, Hirth L (1982) Eur J Biochem 126: 489–494
Walters DA, Vetsch CS, Potts DE, Ludquist RC (1992) Plant Mol Biol 18: 189–200
Warren CA (1994) in the maize handbook. Freeling M, Walbot V, Editors. Springer Laboratory.pp 534–535
Wu FS, Cahoun AB (1995) Plant Science 104: 201–214
Author information
Authors and Affiliations
Additional information
Communicated by A. M. Boudet
Rights and permissions
About this article
Cite this article
Sabri, N., Pelissier, B. & Teissie, J. Transient and stable electrotransformations of intact black Mexican sweet maize cells are obtained after preplasmolysis. Plant Cell Reports 15, 924–928 (1996). https://doi.org/10.1007/BF00231589
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00231589