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Microcallus formation from leaf mesophyll protoplasts in the genus Actinidia Lindl

Plant Cell Reports volume 15, pages 896–899 (1996)Cite this article

Summary

Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, agarose disc type culture for A. arguta cv. Issaï and A. deliciosa and liquid agarose bead type- and disc type culture for A. kolomikta and A. polygama. Several factors influencing purification, browning, survival and sustained division of the protoplasts are briefly discussed.

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Abbreviations

BAP:

benzylaminopurine

CPW:

cell and protoplast washing solution

2,4-D:

dichlorophenoxyacetic acid

GA3:

gibberellic acid

IAA:

indole-3-acetic acid

MES:

2-(N-morpholino) ethanesulfonic acid

NAA:

1-naphthaleneacetic acid

PVP:

polyvinylpyrrolidone

BT:

agarose bead type culture

DT:

agarose disc type culture

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Affiliations

  1. Laboratoire d'Histophysiologie Végétale, URA 1180 CNRS, Université Pierre et Marie Curie, 12 rue Cuvier, F-75005, Paris, France

    Xing-Guo Xiao & Anne-Marie Hirsch

Authors
  1. Xing-Guo Xiao
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  2. Anne-Marie Hirsch
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Communicated by G. Pelletier

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Xiao, XG., Hirsch, AM. Microcallus formation from leaf mesophyll protoplasts in the genus Actinidia Lindl. Plant Cell Reports 15, 896–899 (1996). https://doi.org/10.1007/BF00231583

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  • DOI: https://doi.org/10.1007/BF00231583

Key-words

  • Actinidia arguta
  • A. deliciosa
  • A. kolomikta
  • A. polygama
  • kiwifruit
  • mesophyll protoplast microcallus