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Formation of terpenoid products in Ginkgo biloba L. cultivated cells

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Abstract

Ginkgolides are diterpenes arising from the terpenoid precursor: geranylgeranyl pyrophosphate (GGPP). Incorporation of [1-14C] isopentenylpyrophosphate ([1-14C]IPP) into GGPP was monitored throughout the cultivation cycle of G. biloba L. cultivated cells. Because incorporation of [1-14C]IPP into GGPP had never been monitored in G. biloba, in either the whole plant or cultivated cell system, modifications to existing protocols were necessary. Modifications consisted of extracting the cells with an extraction buffer supplemented with Triton-X-100. Farnesylpyrophosphate (FPP) was the major product formed. The amount of GGPP detected was about one tenth that of FPP.

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Abbreviations

CHAPS:

3-[(3-cholamidopropyl) dimethyl ammonio]-1-propane-sulphonate

DTT:

[1-4 dithiothreitol]

FPP:

farnesylpyrophosphate

GGPP:

geranylgeranylpyrophosphate

IPP:

[1-14C] isopentenylpyrophosphate

PVPP:

polyvinylpolypyrrolidone

Tris:

Tris(hydroxymethyl)aminomethane

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Author notes

  1. Danielle-Julie Carrier

    Present address: Plant Biotechnology Institute/NRC, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada

  2. Jean Archambault

    Present address: Department of Engineering, Université du Québec à Trois-Rivières, G9A 5H7, Trois-Rivières, Québec, Canada

Authors and Affiliations

  1. Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Danielle-Julie Carrier, Jean Archambault, Robert van der Heijden & Robert Verpoorte

Authors
  1. Danielle-Julie Carrier
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  2. Jean Archambault
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  3. Robert van der Heijden
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  4. Robert Verpoorte
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Communicated by F. Constabel

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Carrier, DJ., Archambault, J., van der Heijden, R. et al. Formation of terpenoid products in Ginkgo biloba L. cultivated cells. Plant Cell Reports 15, 888–891 (1996). https://doi.org/10.1007/BF00231581

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  • DOI: https://doi.org/10.1007/BF00231581

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