Summary
An autoradiographical study of the distribution of fast transported material in the cerebro-visceral connective of Anodonta cygnea (L.) following the injection of 3H-5-hydroxytryptophan into the cerebral ganglion has been carried out at both the light- and electronmicroscope levels. Statistical analysis of the results at the electron microscope level revealed that the distribution of grains within the tissues of the cerebro-visceral connective was highly non-random. Both the “circle analysis” and the “hypothetical grain density analysis” methods showed that most of the 3H-activity was associated with dense core vesicles (1000 Å diameter). These dense core vesicles were most frequently seen in the neurosecretory axons found near the periphery of the connective, but a number of labelled vesicles were also present in the small axons. The significance of these findings in relation to the distribution and the fast axonal transport of 5-hydroxytryptamine in the cerebro-visceral connective is discussed.
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We would like to thank Mr. Nigel Cooper for his advice on autoradiographic techniques, Dr. Brij Gupta for helpful discussions on many aspects of this work and Dr. Nancy Lane for review of the manuscript.
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Howes, E.A., McLaughlin, B.J. & Heslop, J.P. The autoradiographical association of fast transported material with dense core vesicles in the central nervous system of Anodonta cygnea (L.). Cell Tissue Res. 153, 545–558 (1974). https://doi.org/10.1007/BF00231546
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DOI: https://doi.org/10.1007/BF00231546