Summary
Changes in subcellular structures of arcuate neurons correlated in a consistent way with stages of the estrous cycle of the rat. Associated with the rough endoplasmic reticulum short ribbons of moderately electron dense material appeared at metestrus and circular or elliptical bodies termed “ribbon-rolls” at diestrus and proestrus. Although present in proestrus, the ribbon-rolls were smaller at this stage. In a few neurons in diestrous females and in ovariectomized animals one to seven months before perfusion multiple large ribbon-rolls occupied much of the cytoplasm. Also, frequency of dense granules and lysosomes increased in diestrus. The significance of the ribbon-rolls and changes in other structures during the estrous cycle are discussed.
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Adamo, N. J.: Ultrastructural features of the lateral preoptic area, median eminence and arcuate nucleus of the rat. Z. Zellforsch. 127, 483–491 (1972)
Andersen, H., van Bullow, F. A., Mollgard, K.: The early development of the pars distalis of human foetal pituitary gland. Z. Anat. Entwickl.-Gesch. 135, 117–138 (1971)
Bjersing, L.: On the ultrastructure of granulosa lutein cells in porcine corpus luteum. Z. Zellforsch. 82, 187–211 (1967)
Blanchette, E. J.: Ovarian steroid cells, II. The lutein cell. J. Cell Biol. 31, 517–542 (1966)
Brawer, J. R.: The role of the arcuate nucleus in the brain-pituitary-gonad axis. J. comp. Neurol. 143, 411–446 (1971)
Brawer, J. R.: The fine structure of the ependymal tanycytes at the level of the arcuate nucleus. J. comp. Neurol. 145, 25–42 (1972)
Bucana, C. D., Nadakavukaren, M. J.: Ultrastructural investigation of the postnatal development of the hamster Harderian gland. Z. Zellforsch. 142, 1–12 (1973)
Carr, I., Carr, J.: Membranous whorls in the testicular interstitial cell. Anat. Rec. 144, 143–147 (1962)
Castel, M.: Ultrastructure of the anuran pars intermedia following severance of hypothalamic connection. Z. Zellforsch. 131, 545–557 (1972)
Chowers, I., McCann, S. M.: Content of luteinizing hormone-releasing factor and luteinizing hormone during the estrous cycle and after changes in gonadal steroid titers. Endocrinology 76, 700–708 (1965)
Christensen, A. K.: The fine structure of testicular interstitial cells in guinea pigs. J. Cell Biol. 26, 911–935 (1965)
Christensen, A. K., Fawcett, D. W.: The fine structure of testicular interstitial cells in mice. Amer. J. Anat. 118, 551–572 (1966)
Curgy, J. J., Anderson, W. A.: Synthèse d'ARN dans le chondriome au cours de la spermiogenèse chez la Drosophile. Z. Zellforsch. 125, 31–44 (1972)
Dubois, P., Girod, C.: Formations lamellaires concentriques dans des cellules antéhypophysaires chez le hamster doré. Z. Zellforsch. 115, 196–211 (1971)
Ford, D. H., Voeller, K., Callegari, B.: Changes in neurons of the median eminence-arcuate region of rats induced by chronic morphine treatment: an electron microscopic study. Anat. Rec. 175, 321 (1973)
Fuxe, K., Hökfelt, T.: Catecholamines in the hypothalamus and the pituitary gland. In: Frontiers in neuroendocrinology (Ganong, W. F. and Martini, L., eds.), p. 47–96. New York: Oxford Univ. Press 1969
Fuxe, K., Hökfelt, T., Jonsson, G.: The effect of gonadal steriods on the tubero-infundibular dopamine neurons. Hormonal steroids (Proceedings of the Third International Congress), p. 806–813 (1970)
Fuxe, K., Hökfelt, T., Nilsson, O.: Activity changes in the tuberoinfundibular dopamine neurons of the rat during various stages of the reproductive cycle. Life Sci. 6, 2057–2061 (1967)
Fuxe, K., Hökfelt, T., Nilsson, O.: Effect of constant light and androgen-sterilization on the amine turnover of the tuberoinfundibular dopamine neurons: blockade of cyclic activity and induction of a persistent high dopamine turnover in the median eminence. Acta endocr. (Kbh.) 69, 625–639 (1972)
Halász, B., Gorski, R. A.: Gonadotrophic hormone secretion in female rats after partial or total interruption of neural afferents to the media basal hypothalamus. Endocrinology 80, 608–622 (1967)
Halász, B., Pupp, L.: Hormone secretion of the anterior pituitary gland after physical interruption of all nervous pathways to the hypophysiotropic area. Endocrinology 77, 553–562 (1965)
Halász, B., Pupp, L., Uhlarik, S.: Hypophysiotrophic area in the hypothalamus. J. Endocr. 25, 147–154 (1962)
Herman, L., Fitzgerald, P. J.: Restitution of pancreatic acinar cells following ethionine. J. Cell Biol. 12, 297–312 (1962)
Hu, F.: Ultrastructural changes in the cell cycle of cultured melanoma cells. Anat. Rec. 170, 41–55 (1971)
Jones, A. L., Fawcett, D. W.: Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in the liver). J. Histochem. Cytochem. 14, 215–232 (1966)
Jong-Brink, M.: The effects of desiccation and starvation upon the weight, histology, and ultrastructure of the reproductive tract of Biomphalaria glabrata, intermediate host of Schistosoma mansoni. Z. Zellforsch. 136, 229–262 (1973)
Keller, P. J., Lichtensteiger, W.: Stimulation of tubero-infundibular dopamine neurons and gonadotrophin secretion. J. Physiol. (Lond.) 219, 385–401 (1971)
Kishi, K.: Fine structural and cytochemical observations on cytoplasmic neucleolus-like bodies in nerve cells of rat medulla oblongata. Z. Zellforsch. 132, 523–532 (1972)
Kumegawa, M., Cattoni, M., Rose, G. G.: Electron microscopy of oral cells in vitro II. Subsurface and intracytoplasmic confronting cisternae in strain KB cells. J. Cell Biol. 36, 443–452 (1968)
Le Beux, Y. J.: An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area. I. Cytoplasmic inclusions resembling nucleoli. Z. Zellforsch. 114, 404–440 (1971)
Lichtensteiger, W.: Cyclic variations of catecholamine content in hypothalamic nerve cells during the estrous cycle of the rat with a concomitant study of the substantia nigra. J. Pharmacol. exp. Ther. 165, 204–215 (1969)
Lisk, R. D., Newlon, M.: Estradiol: Evidence for its direct effect on hypothalamic neurons. Science 139, 223–224 (1963)
Litteria, M.: In vivo alterations in the incorporation of 3H lysine into the medial preoptic nucleus and specific hypothalamic nuclei during the estrous cycle of the rat. Brain Res. 55, 234–237 (1973)
Nickerson, P. A.: Effects of ACTH on membranous whorls in the adrenal gland of the mongolian gerbil. Anat. Rec. 166, 479–490 (1970)
Nickerson, P. A., Curtis, J. C.: Concentric whorls of rough endoplasmic reticulum in adreno-cortical cells of the mongolian gerbil. J. Cell Biol. 40, 859–862 (1969)
Oksche, A., Zimmermann, P., Oehmke, H.-J.: Morphometric studies of tubero-eminential systems controlling reproductive functions. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971 (K. M. Knigge, D. E. Scott, A. Weindl, eds.), p. 142–153. Basel: Karger 1972
Palay, S. L., McGee-Russell, S. M., Gordon, S., Grillo, M. A.: Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide. J. Cell Biol. 12, 385–410 (1962)
Pevet, P., Saboureau, M.: L'épiphyse du Herisson (Erinaceus europaeus L.) male. Z. Zellforsch. 143, 369–385 (1973)
Ramirez, V. D., Sawyer, C. H.: Fluctuations in LH-RF (Luteinizing Hormone Releasing Factor) during the rat estrous cycle. Endocrinology 76, 282–289 (1965)
Rasmussen, S. W.: Ultrastructural studies of spermatogenesis in Drosophila melanogaster Meigen. Z. Zellforsch. 140, 125–144 (1973)
Stoechart, R., Kreike, A. J., Jansen, H. G.: Sizes of granular vesicles in the rat median eminence, with special reference to the zona granulosa. Z. Zellforsch. 146, 501–515 (1973)
Yagi, K.: Changes in firing rates of single preoptic and hypothalamic units following an intravenous administration of estrogen in the castrated female rat. Brain Res. 53, 343–352 (1973)
Zambrano, D.: The arcuate complex of the female rat during the sexual cycle. An electron microscopic study. Z. Zellforsch. 93, 560–570 (1969)
Zambrano, D., de Robertis, D.: The effect of castration upon the ultrastructure of the rat hypothalamus. II Arcuate nucleus and outer zone of the median eminence. Z. Zellforsch. 87, 409–421 (1968)
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Acknowledgments. This investigation was supported by: 1) predoctoral fellowship from the National Institute of Mental Health to J.E.K. 2) NIH-(7-RO1-NS-11650-02) to T. H.W. 3) HD 00867-10 to A.A.G.
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King, J.C., Williams, T.H. & Gerall, A.A. Transformations of hypothalamic arcuate neurons. Cell Tissue Res. 153, 497–515 (1974). https://doi.org/10.1007/BF00231543
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DOI: https://doi.org/10.1007/BF00231543