Summary
Electron microscopy of the dermis of the 9-banded armadillo, Dasypus novemcinctus, reveals an intimate relationship of mast cells to peripheral nerves. Mast cells are routinely found in the dermis in close proximity to nerves, and mast cells are also present within the perineurial cell sheath in the endoneurial space proper. These cells contain electron opaque granules and exhibit numerous surface folds as is characteristic of other species. The degranulation of the mast cells by injection of 0.5% aqueous trypan blue reveals sequential exocytosis of granules, similar to that described for the rat. Degranulation occurs in most dermal and endoneurial mast cells following a single intradermal injection of trypan blue. A small number of mast cells partially degranulate and a few exhibit no degranulation, suggesting a heterogeneous population. It is also noted that mitochondrial morphology differs in degranulating cells, in that they become swollen and vacuolated. The results of this study are discussed in light of previous results reported for other species, mainly at the light microscope level.
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References
Akert, K., Sandri, C., Weibel, E.R., Peper, K., Moor, H.: The fine structure of the perineurial endothelium. Cell Tiss. Res. 165, 281–295 (1976)
Gamble, H.J.: Comparative electron microscopic observations on the connective tissues of a peripheral nerve and a spinal nerve root in the rat. J. Anat. (Lond.) 98, 17–25 (1964)
Heine, H., Förester, F.J.: Relationships between mast cells and preterminal nerve fibers. Z. mikr. anat. Forsch. 89, 934–937 (1975)
Heine, H., Förester, F.J., Schaeg, G.: Zur Histophysiologie der vegetativen Peripherie. Unter besonderer Berücksichtigung der Mastzellen. Z. mikr. anat. Forsch. 89, 948–960 (1975)
Jirge, S.K., Catalano, R.A., Lillie, R.D.: Some histochemical reactions of mast cells of gerbils, hogs, armadillos and cats. Histochemistry 40, 129–135 (1974)
Kovacs, P., Csaba, Gy.: Evidence of the heterogeneity of mast cell population based on their biogenic amine content. Acta morph. Acad. Sci. hung. 25, 9–18 (1977)
Norrby, K., Enerbáck, L., Franzén, L.: Mast cell activation and tissue cell proliferation. Cell Tiss. Res. 170, 289–303 (1976)
Olsson, Y.: The effect of the histamine liberator compound 48/80 on mast cells in normal peripheral nerves. Acta path. microbiol. scand. 68, 563–574 (1966)
Olsson, Y.: Mast cells in the nervous system. Int. Rev. Cytol. 24, 27–70 (1968)
Riley, J.F., West, G.B.: Tissue mast cells: Studies with a histamine-liberator of low toxicity (compound 48/80). J. Path. Bact. 69, 269–282 (1955)
Röhlich, P., Anderson, P., Uvnäs, B.: Electron microscope observations on compound 48/80 — Induced degranulation in rat mast cells. J. Cell Biol. 51, 465–483 (1971)
Rosenthal, S.R.: Histamine as the chemical mediator for cutaneous pain. J. invest. Derm. 69, 98–105 (1977)
Thomas, P.K.: The connective tissue of peripheral nerve: an electron microscope study. J. Anat. (Lond.) 97, 35–44 (1963)
Yoshizumi, M.O., Kirchleimer, W.F., Asbury, A.K.: A light and electron microscopic study of peripheral nerves in an armadillo with disseminated leprosy. Int. J. Leprosy 42, 251–259 (1974)
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Supported by institutional grant from the L.S.U. School of Dentistry
The author thanks Ivis Insua and Angela Pepin for their technical and secretarial assistance and Garbis Kerimian for his photographic work
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Dyer, R.F. Endoneurial mast cells in peripheral nerves of the armadillo dermis. Cell Tissue Res. 192, 97–105 (1978). https://doi.org/10.1007/BF00231026
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DOI: https://doi.org/10.1007/BF00231026