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Properties of inwardly rectifying K+ channels in ventricular myocytes

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Summary

The voltage- and time-dependent properties of whole-cell, multi-channel (outside-out), and single channel inwardly-rectifying K+ currents were studied using adult and neonatal rat, and embryonic chick ventricular myocytes. Inward rectification of the current-voltage relationship was found in the whole-cell and single channel measurements. The steady-state single channel probability of opening decreased with hyperpolarization from EK, as did the mean open time, thereby explaining the time-dependent inactivation of the macroscopic current. Myocytes dialysed with a Mg++-free K+ solution (to remove the property of inward rectification) displayed a quasi-linear current-voltage relationship. The outward K+ currents flowing through the modified inward rectifier channels were able to be blocked by the local anesthetic and anti-arrhythmic agent, lidocaine.

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References

  1. Noble DA: The initiation of the heartbeat. Oxford Press, 1979

  2. Sakmann B, Trube G: Conductance properties of single inwardly rectifying potassium channels in ventricular cells from guinea pig. J Physiol 347:641–657, 1984a

    Google Scholar 

  3. Sakmann B, Trube G: Voltage-dependent inactivation of inward rectifying single channel currents in the guinea pig heart cell membrane. J Physiol 347:659–683, 1984b

    Google Scholar 

  4. Josephson IR, Brown AM: Inwardly rectifying single channel and whole cell K+ currents in rat ventricular myocytes. J Membr Biol 94:19–35, 1986

    Google Scholar 

  5. Mastuda H, Saigusa A, Irisawa H: Ohmic conductance through the inwardly rectifying K channel and blocking by internal Mg++. Nature 325:156–159, 1987

    Google Scholar 

  6. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth, FJ: Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflug Arch 391:85–100, 1981

    Google Scholar 

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Author notes

  1. Ira R. Josephson

    Present address: Department of Physiology and Biophysics, University of Cincinnati, College of Medicine, 45267-0576, Cincinnati, OH, USA

Authors and Affiliations

  1. Department of Physiology and Biophysics, Wright State University, Dayton, Ohio, USA

    Ira R. Josephson

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  1. Ira R. Josephson
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Josephson, I.R. Properties of inwardly rectifying K+ channels in ventricular myocytes. Mol Cell Biochem 80, 21–26 (1989). https://doi.org/10.1007/BF00231000

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  • DOI: https://doi.org/10.1007/BF00231000

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