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Adipose cell differentiation in culture

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Summary

The isolation of preadipocyte cell strains from adipose tissue and from bone marrow, and the establishment of preadipocyte cell lines from embryonic and adult mouse, have been useful tools to study the process of adipose cell differentiation.

This process is regulated both by extracellular signals present in serum and by intracellular signals; the characterization of these signals is under investigation. During adipose cell differentiation morphological and enzymatic changes are dramatic and they are accompanied by qualitative and quantitative variations of the cell protein content. These changes include the induction of the enzymes of the fatty acid and triglyceride synthesizing pathways and a subsequent triglyceride accumulation. The development of hormonal responses to insulin and to β-adrenergics is also observed, and differentiated adipose cells behave essentially like mature adipocytes isolated from adipose tissue.

The present review will be devoted to the main events of adipose conversion in cell lines and cell strains, and to current work which concerns the identification of the triggering signals possibly involved in that process.

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Abbreviations

LPL:

lipoprotein lipase

MGL:

monoglyceride lipase

T3:

triiodothyronine

dbcAMP:

dibutyryl-CAMP

MIX:

1-methyl-3-isobutylxanthine

PG:

prostaglandins

FCS:

fetal calf serum

EDGF:

eye-derived growth factor

PDGF:

platelet-derived growth factor

FGF:

fibroblast growth factor

VLDL:

very low density proteins

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  1. Centre de Biochimie (CNRS LP 73-00), Université de Nice, Parc Valrose, 06034, Nice, France

    G. Ailhaud

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Ailhaud, G. Adipose cell differentiation in culture. Mol Cell Biochem 49, 17–31 (1982). https://doi.org/10.1007/BF00230992

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