Skip to main content
SpringerLink
Log in

Acylation of 1-palmitoyl-sn-glycerophosphocholine by chick brain microsomes is unaffected by fatty acid binding protein

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Rates of incorporation of exogenously supplied fatty acids into 1-palmitoyl-sn-glycerophosphocholine were measured using the microsomal fraction from brains of 14–15 day old chick embryos. The substrate preferences for reacylation were: 18: 2(n − 6) = 20: 4(n − 6) ≥ 20: 5(n − 3) = 18: 3(n − 3) ≥ 18 : 1(n − 9) ≥ 22: 6(n − 3) ≥ 18: 0. The normalized rate with 18: 0 was significantly lower than all other rates except for 22: 6(n − 3), and the acylation rate with 22: 6(n − 3) was significantly lower than with 18: 2(n − 6) and 20: 5(n − 3). With the addition of fatty acid binding protein partially purified from brain cytosol, a decrease (not significant) in the rate of incorporation was observed; the substrate preference was unchanged. In the presence of FABP, normalized rates with 18: 2(n − 6) were significantly higher than with 18: 0, 18: 1(n − 9), or 22: 6(n − 3); rates with 20: 4(n - 6) were significantly higher than those with 22: 6(n − 3). Preliminary data on the acylation of 1-palmitoyl-sn-glycerophosphoethanolamine showed lower rates of incorporation than for the choline analogue and no clear substrate preference, but a similar lack of effect of fatty acid binding protein. These results do not support the proposed function of fatty acid binding protein in the establishment of a phospholipid composition rich in polyunsaturated fatty acids. The results are consistent, however, with the role of the reacylation reaction in the continual turnover of particular substrates [18: 2(n − 6) and 20: 4(n − 6)] used to generate second messengers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GPC:

Glycerophosphocholine

GPE:

Glycerophosphoethanolamine

LPC:

1-palmitoyl-sn-glycerophosphocholine

LPE:

1-palmitoyl-sn-glycerophosphoethanolamine

PUFA:

Polyunsaturated Fatty Acids

FABP:

Fatty Acid Binding Protein

References

  1. Sastry PS: Lipids of nervous tissue: Composition and metabolism. Prog Lipid Res 24: 69–176, 1985

    Google Scholar 

  2. White DA: The phospholipid composition of mammalian tissues. In: GB Ansell, JN Hawthorne, RMC Dawson (eds.) Form and Function of Phospholipids. Elsevier Scientific Publishing Co, Amsterdam, The Netherlands, 1973, pp 441–482

    Google Scholar 

  3. Neuringer M, Anderson GJ, Connor WE: The essentiality of n-3 fatty acids for the development and function of the retina and brain. Ann Rev Nutr 8: 517–541, 1988

    Google Scholar 

  4. Tinoco J, Babcock R, Hincenbergs I, Medwadowski B, Miljanich P: Linolenic acid deficiency: Changes in fatty acid patterns in female and male rats raised on a linolenic acid-deficient diet for two generations. Lipids 13: 6–17, 1978

    Google Scholar 

  5. Dyer JR, Greenwood CE: Neural 22-carbon fatty acids in the weanling rat respond rapidly and specifically to a range of dietary linoleic to α-linolenic fatty acid ratios. J Neurochem 56: 1921–1931, 1991

    Google Scholar 

  6. Neuringer M, Connor WE, Lin DS, Barstad L, Luck S: Biochemical and functional effects of prenatal and postnatal ω3 fatty acid deficiency on retina and brain in rhesus monkeys. Proc Natl Acad Sci USA 83: 4021–4025, 1986

    Google Scholar 

  7. Fisher SK, Doherty FJ, Rowe CE: Deacylation and acylation of phospholipids in nervous tissue. In: LA Horrocks, JN Kanfer, G Porcellati (eds.) Phospholipids in the Nervous System, Vol. 1. Raven Press, New York, 1982, pp 63–74

    Google Scholar 

  8. Bazán HEP, Sprecher H, Bazán NG: De novo biosynthesis of docosahexaenoyl-phosphatidic acid in bovine retinal microsomes. Biochim Biophys Acta 796: 11–19, 1984

    Google Scholar 

  9. Bazán HEP, Bazan NG: Metabolism of docosahexaenoyl groups in phosphatidic acid and in other phospholipids of the retina. In: LA Horrocks, JN Kanfer, G Porcellati (eds.) Phospholipids in the Nervous System, Vol. 2. Raven Press, New York, 1985, pp 209–217

    Google Scholar 

  10. Lands WEM: Metabolism of glycerolipids. II. The enzymatic acylation of lysolecithin. J Biol Chem 235: 2233–2237, 1960

    Google Scholar 

  11. Webster GR, Alpern RJ: Studies on the acylation of lysolecithin by rat brain. Biochem J 90: 35–42, 1964

    Google Scholar 

  12. Woelk H, Porcellati G: Investigations on the lipid turnover of brain tissue at a cellular and subcellular level. In: LA Horrocks, JN Kanfer, G Porcellati (eds.) Phospholipids in the Nervous System, Vol 1. Raven Press, New York, 1982, pp 105–110

    Google Scholar 

  13. Bass NM: The cellular fatty acid binding proteins: Aspects of structure, regulation, and function. Int Rev Cytol 111: 143–184, 1988

    CAS  PubMed  Google Scholar 

  14. Paulussen RJA, Veerkamp JH: Intracellular fatty-acid-binding proteins: Characteristics and function. In: HJ Hilderson (ed.) Subcellular Biochemistry, Vol 16. Plenum Press, New York, 1990, pp 175–226

    Google Scholar 

  15. Matarese V, Stone RL, Waggoner DW, Bernlohr DA: Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 28: 245–272, 1989

    Google Scholar 

  16. Glatz JFC, van der Vusse GJ: Cellular fatty acid-binding proteins: Current concepts and future directions. Mol Cell Biochem 98: 237–251, 1990

    Google Scholar 

  17. Schoentgen F, Bonanno LM, Pignéde G, Jollés P: Amino acid sequence and some ligand binding properties of fatty acid-binding protein from bovine brain. Mol Cell Biochem 98: 35–39, 1990

    Google Scholar 

  18. Schoentgen F, Pignede G, Bonanno LM, Jollès P: Fatty-acid-binding protein from bovine brain: Amino acid sequence and some properties. Eur J Biochem 185: 35–40, 1989

    Google Scholar 

  19. Veerkamp JH, Paulussen RJA, Peeters RA, Maatman RGHJ, van Moerkerk HTB, van Kuppevelt THMSM: Detection, tissue distribution and (sub)cellular localization of fatty acid-binding protein types. Mol Cell Biochem 98: 11–18, 1990

    Google Scholar 

  20. Kaikaus RM, Bass NM, Ockner RK: Functions of fatty acid binding proteins. Experientia 46: 617–630, 1990

    Google Scholar 

  21. Dreyfus H, Urban PF, Edel-Harth S, Mandel P: Developmental pattern of gangliosides and of phospholipids in chick retina and brain. J Neurochem 25: 245–250, 1975

    Google Scholar 

  22. Anderson GJ, Connor WE, Corliss JD, Lin DS: Rapid modulation of the n − 3 docosahexaenoic acid levels in the brain and retina of the newly hatched chick. J Lipid Res 30: 433–441, 1989

    Google Scholar 

  23. Lowry OH, Rosebrough JN, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    CAS  PubMed  Google Scholar 

  24. Folch J, Lees M, Sloane-Stanley GHS: A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226: 497–509, 1957

    CAS  PubMed  Google Scholar 

  25. Sellner PA, Phillips AR: Phospholipid synthesis by chick retinal microsomes: fatty acid preference and effect of fatty acid binding protein. Lipids 26: 62–67, 1991

    Google Scholar 

  26. Tacconi M, Wurtman RJ: Phosphatidylcholine produced in rat synaptosomes by N-methylation is enriched in polyunsaturated fatty acids. Proc Natl Acad Sci USA 82: 4828–4831, 1985

    Google Scholar 

  27. Lakher MB, Wurtman RJ: Molecular composition of the phosphatidylcholines produced by the phospholipid methylation pathway in rat brain in vivo. Biochem J 244: 325–330, 1987

    Google Scholar 

  28. Badiani K, Arthur G: 2-acyl-sn-glycero-3-phosphoethanolamine lysophospholipase A2 activity in guinea-pig heart microsomes. Biochem J 275: 393–398, 1991

    Google Scholar 

  29. Raben DM, Pessin MS, Rangan LA, Wright TM: Kinetic and molecular species analyses of mitogen-induced increases in diglycerides: Evidence for stimulated hydrolysis of phosphoinositides and phosphatidylcholine. J Cell Biochem 44: 117–125, 1990

    Google Scholar 

  30. Anderson GJ, Connor WE: Uptake of fatty acids by the developing rat brain. Lipids 23: 286–290, 1988

    Google Scholar 

  31. Bordewick U, Heese M, Börchers T, Robenek H, Spener F: Compartmentation of hepatic fatty-acid-binding protein in liver cells and its effect on microsomal phosphatidic acid biosynthesis. Biol Chem Hoppe-Seyler 370: 229–238, 1989

    Google Scholar 

  32. Reddy TS, Bazán NG: Long-chain acyl CoA synthetase in microsomes from rat brain gray matter and white matter. Neurochem Res 10: 377–386, 1985

    Google Scholar 

  33. Leray C, Pelletier A, Massarelli R, Dreyfus H, Freysz L: Molecular species of choline and ethanolamine phospholipids in rat cerebellum during development. J Neurochem 54: 1677–1681, 1990

    Google Scholar 

  34. Sellner PA, Clough JA: Fatty acid composition of phospholipids from chick neural retina during development. Exp Eye Res 54: 725–730, 1992

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, and Department of Ophthalmology, University of Kansas Medical Center, 66160, Kansas City, Kansas, USA

    Peggy A. Sellner & Arlana R. Phillips

Authors
  1. Peggy A. Sellner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arlana R. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sellner, P.A., Phillips, A.R. Acylation of 1-palmitoyl-sn-glycerophosphocholine by chick brain microsomes is unaffected by fatty acid binding protein. Mol Cell Biochem 117, 119–125 (1992). https://doi.org/10.1007/BF00230750

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00230750

Key words

Search

Navigation