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The problem of the stability of globular proteins

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Summary

The article gives a survey on protein stability. Starting out from approaches for stability measurement which are based on the determination of Gibbs energy change in protein unfolding by denaturants, protonation, heat, scanning calorimetry, and hydrogen exchange, their implications such as reversibility, completeness of unfolding and the two-state assumption are dealt with.

A data compilation of Gibbs energy change in unfolding of different proteins is given. The data, which for the most part range between 25 and 60 kJ mol−1, are discussed in terms of protein functioning, turnover, and structural properties. Phase diagrams are proposed in order to realize a more comprehensive thermodynamic treatment of proteins.

Factors which contribute to protein stability are summarized. The paper includes the thermodynamic principles of protein stability as well as special studies on proteolytic fragments, amino acid replacements, cross links, prosthetic groups, and ions which contribute to protein stability.

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References

  1. Anson, M. L., 1945. Adv. Protein Chem. 2, 361–386.

    Google Scholar 

  2. Putnam, F. W., 1953. The Proteins (Neurath, H. and Bailey, K., eds.) Vol. 1B, Academic Press, New York, pp. 807–892.

    Google Scholar 

  3. Kauzmann, W., 1959. Adv. Protein Chem. 14, 1–63.

    Google Scholar 

  4. Joly, M., 1965. A Physico-Chemical Approach to the Denaturation of Proteins, Academic Press, London and New York.

    Google Scholar 

  5. Tanford, C., 1968. Adv. Protein Chem. 23, 121–282.

    Google Scholar 

  6. Tanford, C., 1970. Adv. Protein Chem. 24, 1–95.

    Google Scholar 

  7. Lumry, R. & Biltonen, R., 1969. Structure and Stability of Biological Macromolecules (Timasheff, S. N. and Fasman, G., eds.), Marcel Dekker Inc., New York, pp. 65–212.

    Google Scholar 

  8. Brandts, J. F., 1969. Structure and Stability of Biological Macromolecules (Timasheff, S. N. and Fasman, G., eds.), Marcel Dekker, Inc., New York, pp. 213–290.

    Google Scholar 

  9. Kuntz, I. D. & Kauzmann, W., 1974. Adv. Protein Chem. 28,239–345.

    Google Scholar 

  10. Franks, F. & Eagland, D., 1975. Crit. Rev. Biochem. 3, 165–219.

    Google Scholar 

  11. Pace, C. N., 1975. Crit. Rev. Biochem. 3, 1–43.

    Google Scholar 

  12. Edelhoch, H. & Osborne, J., J.C., 1976. Adv. Protein Chem. 30, 183–250.

    Google Scholar 

  13. Lapanje, S., 1978. Physicochemical Aspects of Protein Denaturation, John Wiley & Sons, New York.

    Google Scholar 

  14. Pfeil, W. & Privalov, P. L., 1979. Biochemical Thermodynamics, Studies in Modern Thermodynamics Vol. 1(Jones, M. N., ed.), Elsevier Scientific Publishing Company, Amsterdam-Oxford-New York, pp. 75–115.

    Google Scholar 

  15. Privalov, P. L., 1979. Adv. Protein Chem. 33, 167–241.

    Google Scholar 

  16. Anfinsen, C. B., 1973. Science 181, 223–230.

    Google Scholar 

  17. Anfinsen, C. B. & Scheraga, H. A., 1975. Adv. Protein Chem. 29, 205–299.

    Google Scholar 

  18. Aune, K. C. & Tanford, C., 1969. Biochemistry 8, 4586–4590.

    Google Scholar 

  19. Greene, Jr., R. F. & Pace, C. N., 1974. J. Biol. Chem. 249, 5388–5393.

    Google Scholar 

  20. Tanford, C., 1964. J. Am. Chem. Soc. 86, 2050–2059.

    Google Scholar 

  21. Hermans, Jr., J. & Scheraga, H. A., 1961. J. Am. Chem. Soc. 83, 3283–3292.

    Google Scholar 

  22. Hermans, Jr, J. & Scheraga, H. A., 1961. J. Am. Chem. Soc. 83, 3293–3300.

    Google Scholar 

  23. Hermans, Jr., J. & Acampora, G., 1967. J. Am. Chem. Soc. 89,1547–1552.

    Google Scholar 

  24. Acampora, G. & Hermans, Jr., J., 1967. J. Am. Chem. Soc. 89,1543–1547.

    Google Scholar 

  25. Pfeil, W. & Privalov, P. L., 1976. Biophys. Chem. 4,23–32.

    Google Scholar 

  26. Privalov, P. L. & Khechinashvili, N. N., 1974. J. Mol. Biol. 86,665–684.

    Google Scholar 

  27. Ackermann, T., 1969. Biochemical Microcalorimetry (Brown, H. D., ed.), Academic Press, New York and London, pp. 121–148.

    Google Scholar 

  28. Privalov, P. L., 1974. FEBS Lett. 40 S, 140–153.

    Google Scholar 

  29. Sturtevant, J. M., 1974. Ann. Rev. Biophys. Bioengng. 3, 35–51.

    Google Scholar 

  30. Wadsö, L, 1974. Pure Appl. Chem. 38, 529–538.

    Google Scholar 

  31. Wadsö, I., 1975. New Techniques in Biophysics and Cell Biology (Pain, R. H. and Smith, B. J., eds.), John Wiley & Sons, London, New York, Sidney, Toronto, pp. 85–126.

    Google Scholar 

  32. Privalov, P. L., Plotnikov, V. V. & Filimonov, V. V., 1975. J. Chem. Thermodynamics 7, 41–47.

    Google Scholar 

  33. Privalov, P. L., 1980. Pure and Appl. Chem. 52, 479–497.

    Google Scholar 

  34. Linderström-Lang, K. U. & Schellman, J. A., 1959. The Enzymes (Boyer, P. D., Lardy, H. & Myrbäck, K., eds.) 2nd ed., Vol. l, Academic Press, New York, pp. 443–510.

    Google Scholar 

  35. Hvidt, A. & Nielsen, S. O., 1966. Adv. Protein Chem. 21, 287–386.

    Google Scholar 

  36. Willumsen, L., 1971. C.R. Trav. Lab. Carlsberg 38, 223–295.

    Google Scholar 

  37. Hvidt, A. & Wallevik, K. J., 1972. J. Biol. Chem. 247, 1530–1535.

    Google Scholar 

  38. Gudkov, A. T., Tumanova, L. G., Venyaminov, S. Yu. & Khechinashvili, N. N., 1978. FEBS-Lett. 93, 215–218.

    Google Scholar 

  39. Khechinashvili, N. N., Koteliansky, V. E., Gogia, Z. V., Littlechild, J. & Dijk, J., 1978. FEBS-Lett. 95, 270–272.

    Google Scholar 

  40. Bendzko, P. & Pfeil, W., in preparation.

  41. Givol, D., DeLorenzo, F., Goldberger, R. F. & Anfinsen, C. B., 1965. Proc. Natl. Acad. Sci. USA 53, 676–684.

    Google Scholar 

  42. Steiner, D. F. & Clark, J. L., 1968. Proc. Natl. Acad. Sci. USA 60, 622–629.

    Google Scholar 

  43. Taniuchi, H. & Anfinsen, C. B., 1969. J. Biol. Chem. 244, 3864–3875.

    Google Scholar 

  44. Ikai, A. & Tanford, C., 1973. J. Mol. Biol. 73, 145–163.

    Google Scholar 

  45. Tanford, C., Aune, K. C. & Ikai, A., 1973. J. Mol. Biol. 73, 185–197.

    Google Scholar 

  46. Creighton, T. E., 1979. J. Mol. Biol. 129, 235–264.

    Google Scholar 

  47. Lumry, R., Biltonen, R. L. & Brandts, J. F., 1966. Biopolymers 4, 917–944.

    Google Scholar 

  48. Tsong, T. Y., Hearn, R. P., Wrathall, D. P. & Sturtevant, J. M., 1970. Biochemistry 9, 2666–2677.

    Google Scholar 

  49. Jackson, W. M. & Brandts, J. F., 1970. Biochemistry 9, 2294–2301.

    Google Scholar 

  50. O'Reilly, J. M. & Karasz, F. E., 1970. Biopolymers 9, 1429–1435.

    Google Scholar 

  51. Velicelebi, G. & Sturtevant, J. M., 1979. Biochemistry 18, 1180–1186.

    Google Scholar 

  52. Pfeil, W., 1981. Biophysical Chemistry 13, 181–186.

    Google Scholar 

  53. feil, W. & Bendzko, P., 1980. Biochim. Biophys. Acta, 626,73–78.

    Google Scholar 

  54. Matthews, C. R., Crisanti, M. M., Gepner, G. L., Velicelebi, G. & Sturtevant, J. M., 1980. Biochemistry 19, 1290–1293.

    Google Scholar 

  55. Jacobson, A. L. & Braun, H., 1977. Biochim. Biophys. Acta 493, 142–153.

    Google Scholar 

  56. Biltonen, R. L. & Freire, E., 1978. CRC Crit. Rev. Biochem. 5, 85–124.

    Google Scholar 

  57. Freire, E. & Biltonen, R. L., 1978. Biopolymers 17, 463–479.

    Google Scholar 

  58. Freire, E. & Biltonen, R. L., 1978. Biopolymers 17, 481–496.

    Google Scholar 

  59. Tiktopulo, E. I. & Privalov, P. L., 1978. FEBS-Lett. 91, 57–58.

    Google Scholar 

  60. Zavyalov, P. V., Troitsky, G. V., Khechinashvili, N. N. & Privalov, P. L., 1977. Biochim. Biophys. Acta 492. 102–111.

    Google Scholar 

  61. Chlebowski, J. F. & Mabrey, S., 1977. J. Biol. Chem. 252, 7042–7052.

    Google Scholar 

  62. DeWitt, J. N. & Swinkels, G. A. M., 1980. Biochim. Biophys. Acta 624, 40–50.

    Google Scholar 

  63. Enomoto, K. & Sato, R., 1973. Biochem. Biophys. Res. Commun. 51, 1–7.

    Google Scholar 

  64. Pabo, C. O., Sauer, R. T., Sturtevant, J. M. & Ptashne, M., 1979. Proc. Natl. Acad. Sci. USA 76, 1608–1612.

    Google Scholar 

  65. Vickers, L. P., Donovan, J. W. & Schachman, H. K., 1978. J. Biol. Chem. 253, 8493–8498.

    Google Scholar 

  66. Chlebowski, J. F., Mabrey, S. & Falk, M. C., 1979. J. Biol. Chem. 254, 5745–5753.

    Google Scholar 

  67. Tsalkova, T. N. & Privalov, P. L., 1980. Biochim. Biophys. Acta 624, 196–204.

    Google Scholar 

  68. Donovan, J. W. & Mihalyi, E., 1974. Proc. Natl. Acad. Sci. USA 71, 4125–4128.

    Google Scholar 

  69. Kuwajima, K., Nitta, K., Yoneyama, M. & Sugai, S., 1976. J. Mol. Biol. 106, 359–373.

    Google Scholar 

  70. Kuwajima, K., 1977. J. Mol. Biol. 114, 241–258.

    Google Scholar 

  71. Sommers, P. B. & Kronman, M. J., 1980. Biophys. Chem. 11, 217–232.

    Google Scholar 

  72. Baldwin, R. L., 1975. Annu. Rev. Biochem. 44, 453–475.

    Google Scholar 

  73. Brandts, J. F., Halverson, H. R. & Brennan, M., 1975. Biochemistry 14, 4953–4963.

    Google Scholar 

  74. Lin, L.-N. & Brandts, J. F., 1978. Biochemistry 17, 4102–4110.

    Google Scholar 

  75. Garel, J.-R., 1980. Proc. Natl. Acad. Sci. USA 77,795–798.

    Google Scholar 

  76. Kanehisa, M. I. & Tsong, T. Y., 1978. J. Mol. Biol. 124, 177–194.

    Google Scholar 

  77. Kanehisa, M. I. & Tsong, T. Y., 1979. Biopolymers 18, 1375–1388.

    Google Scholar 

  78. Aune, K. C., Salahuddin, A., Zarlengo, M. H. & Tanford, C., 1967. J. Biol. Chem. 242, 4486–4489.

    Google Scholar 

  79. Pfeil, W. & Privalov, P. L., 1976. Biophys. Chem. 4, 33–40.

    Google Scholar 

  80. Ahmad, F. & Salahuddin, A., 1974. Biochemistry 13, 245–249.

    Google Scholar 

  81. Sakakibara, R. & Hamaguchi, K., 1968. J. Biochem. (Tokyo) 64, 613–618.

    Google Scholar 

  82. Takase, K., Nitta, K. & Sugai, S., 1974. Biochim. Biophys. Acta 371, 352–359.

    Google Scholar 

  83. Pfeil, W. & Privalov, P. L., 1976. Biophys. Chem. 4, 41–50.

    Google Scholar 

  84. Pace, C. N. & Vanderburgh, K. E., 1979. Biochemistry 18, 288–292.

    Google Scholar 

  85. Nakanishi, M., Tsuboi, M. & Ikegami, A., 1972. J. Mol. Biol. 70, 351–361.

    Google Scholar 

  86. Hvidt, A., 1964. Compt. Rend. Trav. Carlsberg 34, 299–317.

    Google Scholar 

  87. Knox, D. G. & Rosenberg, A., 1980. Biopolymers 19, 1049–1063.

    Google Scholar 

  88. Sophianopoulos, A. J. & Weiss, B. J., 1964. Biochemistry 3, 1920–1928.

    Google Scholar 

  89. Robinson, D. R. & Jencks, W. P., 1965. J. Amer. Chem. Soc. 87, 2462–2470.

    Google Scholar 

  90. Lee, J. C. & Timasheff, S. N., 1974. Biochemistry 13, 257–265.

    Google Scholar 

  91. Salahuddin, A. & Tanford, C., 1970. Biochemistry 9, 1342–1347.

    Google Scholar 

  92. Tanford, C., 1962. J. Am. Chem. Soc. 84, 4240–4247.

    Google Scholar 

  93. Brandts, J. F., 1964. J. Am. Chem. Soc. 86, 4291–4301.

    Google Scholar 

  94. Brandts, J. F., 1964. J. Am. Chem. Soc. 86, 4302–4314.

    Google Scholar 

  95. Ottesen, M., 1971. Methods Biochem. Anal. 20, 135–168.

    Google Scholar 

  96. Tall, A. R., Shipley, G. G. & Small, D. M., 1976. J. Biol. Chem. 251, 3749–3755.

    Google Scholar 

  97. Edelstein, C. & Scann, A. M., 1980. J. Biol. Chem. 255, 5747–5754.

    Google Scholar 

  98. Tall, A. R., Small, D. M., Deckelbaum, R. J. & Shipley, G. G., 1977. J. Biol. Chem. 252, 4701–4711.

    Google Scholar 

  99. Mantulin, W. M., Rohde, M. F., Gotto, Jr., A. M. & Pownall, H. J., 1980. J. Biol. Chem. 255, 8185–8191.

    Google Scholar 

  100. Biltonen, R. L. & Lumry, R., 1969. J. Am. Chem. Soc. 91, 4256–4264.

    Google Scholar 

  101. Greene, R. F., personal communication to Pace, C. N., 104.

  102. Brandts, J. F., personal communication to Pace, C. N., 11.

  103. Bendzko, P. & Pfeil, W., 1980. Acta biol. med. germ. 39, 47–53.

    Google Scholar 

  104. Knapp, J. A. & Pace, C. N., 1974. Biochemistry 13, 1289–1294.

    Google Scholar 

  105. McLendon, G. & Smith, M., 1978. J. Biol. Chem. 253, 4004–4008.

    Google Scholar 

  106. Kawaguchi, H. & Noda, H., 1977. J. Biochem. (Tokyo) 81, 1307–1317.

    Google Scholar 

  107. Nojima, H., Hon-nami, K., Oshima, T. & Noda, H., 1978. J. Mol. Biol. 122,33–42.

    Google Scholar 

  108. Hon-nami, K. & Oshima, T., 1979. Biochemistry 18, 5693–5697.

    Google Scholar 

  109. Holladay, L. A., Hammonds, Jr., R. G. & Puett, D., 1974. Biochemistry 13, 1653–1661.

    Google Scholar 

  110. Rowe, E. S. & Tanford, C., 1973. Biochemistry 12, 4822–4827.

    Google Scholar 

  111. Rowe, E. S., 1976. Biochemistry 15, 905–916.

    Google Scholar 

  112. Kita, N., Kuwajima, K., Nitta, K. & Sugai, S., 1976. Biochim. Biophys. Acta 427. 350–358.

    Google Scholar 

  113. Sugai, S., Yashiro, H. & Nitta, K., 1973. Biochim. Biophys. Acta 328, 35–41.

    Google Scholar 

  114. Takase, K., Nitta, K. & Sugai, S., 1974. Biochim. Biophys. Acta 371, 352–359.

    Google Scholar 

  115. Nozaka, M., Kuwajima, K., Nitta, K. & Sugai, S., 1978. Biochemistry 17, 3753–3758.

    Google Scholar 

  116. Elwell, M. L. & Schellman, J. A., 1977. Biochim. Biophys. Acta 494, 367–383.

    Google Scholar 

  117. McLendon, G., 1977. Biochem. Biophys. Res. Commun. 77, 959–966.

    Google Scholar 

  118. Puett, D., Friebele, E. & Hammonds, Jr., R. G., 1973. Biochim. Biophys. Acta 328, 261–277.

    Google Scholar 

  119. Puett, D., 1973. J. Biol. Chem. 248, 4623–4634.

    Google Scholar 

  120. Schechter, A. N. & Epstein, C. J., 1968. J. Mol. Biol. 567–589.

  121. Abrashi, H., 1970. Compt. Rend. Trav. Lab. Carlsberg 37, 129–144.

    Google Scholar 

  122. Abrashi, H., 1970. Compt. Rend. Trav. Lab. Carlsberg 37, 107–128.

    Google Scholar 

  123. McLendon, G. & Sandberg, K., 1978. J. Biol. Chem. 253, 3913–3917.

    Google Scholar 

  124. Ahmad, F. & Salahuddin, A., 1976. Biochemistry 15, 5168–5195.

    Google Scholar 

  125. Leibman, D. Ya., Tiktopulo, E. I. & Privalov, P. L., 1975. Biofizika 20, 376–379.

    Google Scholar 

  126. Filimonov, V. V., Pfeil, W., Tsalkova, T. N. & Privalov, P. L., 1978. Biophys. Chem. 8, 117–122.

    Google Scholar 

  127. Robson, B. & Pain, R. H., 1976. Biochem. J. 155, 331–344.

    Google Scholar 

  128. Abroad, F. & McPhie, P., 1978. Biochemistry 17, 241–246.

    Google Scholar 

  129. Nojima, H., Ikai, A., Oshima, T. & Noda, H., 1977. J. Mol. Biol. 116, 429–442.

    Google Scholar 

  130. Chen, C.-H., Kao, O. H. W. & Berns, D. S., 1977. Biophys. Chem. 7, 81–86.

    Google Scholar 

  131. Brandts, J. F. & Hunt, L., 1967. J. Am. Chem. Soc. 89, 4826–4838.

    Google Scholar 

  132. Puett, D., 1972. Biochemistry 11, 1980–1990.

    Google Scholar 

  133. Barnard, E. A., 1964. J. Mol. Biol. 10, 235–262.

    Google Scholar 

  134. Richarz, R., Sehr, P., Wagner, G. & Wüthrich, K., 1979. J. Mol. Biol. 130, 19–30.

    Google Scholar 

  135. Oshino, N., 1978. Pharmac. Ther. A 2, 477–515.

    Google Scholar 

  136. Mathews, F. S., Argos, P. & Levine, M., 1971. Gold Spring Harb. Symp. quant. Biol. 36, 387–395.

    Google Scholar 

  137. Mathews, F. S., Levine, M. & Argos, P., 1972. J. Mol. Biol. 64, 449–464.

    Google Scholar 

  138. Dailey, H. A. & Strittmatter, P., 1979. J. Biol. Chem. 254, 5388–5396.

    Google Scholar 

  139. Myer, Y. P., Saturno, A. F., Verma, B. C. & Pande, A., 1979. J. Biol. Chem. 254, 11202–11207.

    Google Scholar 

  140. Watt, G. D. & Sturtevant, J. M., 1969. Biochemistry 8, 4567–4571.

    Google Scholar 

  141. Nakamura, S. & Koga, K., 1977. Biochem. Biophys. Res. Commun. 78, 806–810.

    Google Scholar 

  142. Goldberg, A. L. & Dice, J. F., 1974. Annu. Rev. Biochem. 43, 835–869.

    Google Scholar 

  143. Okunuki, K., 1961. Adv. Enzymol. 23, 29–82.

    Google Scholar 

  144. McLendon, G. & Radany, E., 1978. J. Biol. Chem. 253, 6335–6337.

    Google Scholar 

  145. Pain, R. H., 1978. Characterisation of Protein Conformation and Function (Franks, F., ed.), Symposium Press, London, pp. 19–36.

    Google Scholar 

  146. Wetlaufer, D. B., 1980. Protein Folding (Jaenicke, R., ed.), Elsevier/North Holland Biomed. Press, Amsterdam and New York, pp. 323–329.

    Google Scholar 

  147. Aune, K. C. & Tanford, C., 1969. Biochemistry 8, 4579–4585.

    Google Scholar 

  148. Biltonen, R. L. & Lumry, R., 1965. J. Am. Chem. Soc. 87, 4208–4209.

    Google Scholar 

  149. Hawley, S. A., 1971. Biochemistry 10, 2436–2441.

    Google Scholar 

  150. Zipp, A. & Kauzmann, W., 1973. Biochemistry 12, 4217–4228.

    Google Scholar 

  151. Hawley, S. A., 1978. Methods in Enzymology 49, 14–24.

    Google Scholar 

  152. Brandts, J. F., 1967. Thermobiology (Rose, A. H., ed.), Academic Press, London and New York, pp. 25–72.

    Google Scholar 

  153. Privalov, P. L., Tiktopulo, E.I. & Tischenko, V. M., 1979. J. Mol. Biol. 127, 203–216.

    Google Scholar 

  154. Douzou, P., Debey, P. & Franks, F., 1977. Nature 268, 466.

    Google Scholar 

  155. Puett, D., 1972. Biochemistry 11, 4304–4307.

    Google Scholar 

  156. Puett, D., 1973. J. Biol. Chem. 248, 3566–3572.

    Google Scholar 

  157. Sherwood, L. M. & Potts, Jr., J. T., 1965. J. Biol. Chem. 240, 3799–3805.

    Google Scholar 

  158. Lin, M. C., 1970. J. Biol. Chem. 245, 6726–6731.

    Google Scholar 

  159. Hayashi, R., Moore, S. & Merrifield, R. B., 1973. J. Biol. Chem. 248, 3889–3892.

    Google Scholar 

  160. Hartley, R. W., 1970. Biochem. Biophys. Res. Commun. 40, 263–270.

    Google Scholar 

  161. Creighton, T. E., Dyckes, D. F. & Sheppard, R. C., 1978. J. Mol. Biol. 119, 507–518:

    Google Scholar 

  162. Johnson, E. R., Oh, K.-J. & Wetlaufer, D. B., 1976. J. Biol. Chem. 251, 3154–3157.

    Google Scholar 

  163. Spatz, L. & Strittmatter, P., 1971. Proc. Natl. Acad. Sci. USA 68, 1042–1046.

    Google Scholar 

  164. Visser, L., Robinson, N. C. & Tanford, C., 1975. Biochemistry 14, 1194–1199.

    Google Scholar 

  165. Tajima, S., Enomoto, K. & Sato, R., 1976. Arch. Biochem. Biophys. 172, 90–97.

    Google Scholar 

  166. Tajima, S., Enomoto, K. & Sato, R., 1978. J. Biochem. (Tokyo) 84, 1573–1586.

    Google Scholar 

  167. Warme, P. K. & Scheraga, H. A., 1975. Biochemistry 14, 3509–3517.

    Google Scholar 

  168. Warme, P. K., 1975. Biochemistry 14, 3518–3526.

    Google Scholar 

  169. Argos, P., Rossmann, M. G., Grau, K. M., Zuber, H., Frank, G. & Tratschin, J. D., 1979. Biochemistry 18, 5698–5703.

    Google Scholar 

  170. Bull, H. B. & Breese, K., 1973. Arch. Biochem. Biophys. 156, 681–686.

    Google Scholar 

  171. Stellwagen, E. & Wilgus, H., 1978. Nature 275, 342–343.

    Google Scholar 

  172. Zuber, H. ed. 1978. Enzymes and Proteins from Thermophilic Microorganisms, Birkhdäuser Verlag, Basel and Stuttgart.

    Google Scholar 

  173. Colman, P. M., Jansonius, J. N. & Matthews, B. W., 1972. J. Mol. Biol. 70, 701–724.

    Google Scholar 

  174. Perutz, M. F. & Raidt, H., 1975. Nature 255, 256–259.

    Google Scholar 

  175. Perutz, M. F., 1978. Science 201, 1187–1191.

    Google Scholar 

  176. Grütter, M. G., Hawkes, R. B. & Matthews, B. W., 1979. Nature 277, 667–669.

    Google Scholar 

  177. Stoesz, J. & Lumry, R. W., 1979. Biophys. Chem. 10, 105–112.

    Google Scholar 

  178. Jacobson, A. L. & Braun, H., 1977. Biochim. Biophys. Acta 493. 142–153.

    Google Scholar 

  179. Schellman, J. A., 1955. Compt. Rend. Trav. Lab. Carlsberg 29, 230–259.

    Google Scholar 

  180. Markus, G., 1964. J. Biol. Chem. 239, 4163–4170.

    Google Scholar 

  181. Imoto, T. & Rupley, J. A., 1973. J. Mol. Biol. 80, 657–667.

    Google Scholar 

  182. Johnson, R. E., Adams, P. & Rupley, J. A., 1978. Biochemistry 17, 1479–1489.

    Google Scholar 

  183. Imoto, T., Fukuda, K.-I. & Yagashita, K., 1974. Biochim. Biophys. Acta 336, 264–269.

    Google Scholar 

  184. Flory, P. J., 1956. J. Am. Chem. Soc. 78, 5222–5235.

    Google Scholar 

  185. Poland, D. C. & Scheraga, H. A., 1965. Biopolymers 3, 379–399.

    Google Scholar 

  186. Fukada, H. & Takahashi, K., 1980. J. Biochem. (Tokyo) 87, 1111–1117.

    Google Scholar 

  187. Geisow, M. J., 1977. Nature 269, 9–10.

    Google Scholar 

  188. Schulz, G. E. & Schirmer, R. H., 1979. Principles of Protein Structure, Springer-Verlag, New York.

    Google Scholar 

  189. Creighton, T. E., 1974. J. Mol. Biol. 87, 563–602.

    Google Scholar 

  190. Creighton, T. E., 1974. J. Mol Biol. 87, 603–624.

    Google Scholar 

  191. Creighton, T. E., 1975. J. Mol. Biol. 95, 167–199.

    Google Scholar 

  192. Creighton, T. E., 1977. J. Mol. Biol. 113, 329–341.

    Google Scholar 

  193. Creighton, T. E., 1979. J. Mol. Biol. 129, 411–431.

    Google Scholar 

  194. Creighton, T. E., 1977. J. Mol. Biol. 113, 313–328.

    Google Scholar 

  195. Creighton, T. E., 1980. Protein Folding (Jaenicke, R., ed.), Elsevier/North Holland Biomed. Press, Amsterdam and New York, pp. 427–446.

    Google Scholar 

  196. Pechére, J.-F., Damaille, J. & Capony, J.-P. 1971. Biochim. Biophys. Acta 236, 391–408.

    Google Scholar 

  197. Kretsinger, R. H. & Nockolds, C. E., 1973. J. Biol. Chem. 248, 3313–3326.

    Google Scholar 

  198. Kretsinger, R. H., 1976. Annu. Rev. Biochem. 45, 239–266.

    Google Scholar 

  199. Kretsinger, R. H. & Barry, C. D., 1975. Biochim. Biophys. Acta 405, 40–52.

    Google Scholar 

  200. Hiraoko, Y., Segawa, T., Kuwajima, K., Sugai, S. & Murai, N., 1980. Biochem. Biophys. Res. Commun. 95, 1098–1104.

    Google Scholar 

  201. Wyman, J., Gill, S. J. & Colosimo, A., 1979. Biophys. Chem. 10, 363–369.

    Google Scholar 

  202. Record, Jr., M. T., Anderson, C. F. & Lohman, T. M., 1978. Quart. Rev. Biophys. 11, 103–178.

    Google Scholar 

  203. Donovan, J. W. & Beardslee, R. A., 1975. J. Biol. Chem. 250, 1966–1971.

    Google Scholar 

  204. Donovan, J. W. & Ross, K. D., 1973. Biochemistry 12, 512–517.

    Google Scholar 

  205. Back, J. F., Oakenfull, D. & Smith, M. B., 1979. Biochemistry 18, 5191–5196.

    Google Scholar 

  206. Hägerdal, B., 1976. The Effects of Acetone and Heat Treatments on Myoglobin at Neutral pH, Thesis, Lund.

  207. Koch-Schmidt, A.-C. & Mosbach, K., 1977. Biochemistry 16, 2101–2105.

    Google Scholar 

  208. Koch-Schmidt, A.-C. & Mosbach, K., 1977. Biochemistry 16, 2105–2109.

    Google Scholar 

  209. Richards, F. M., 1977. Annu. Rev. Biophys. Bioengng. 6, 151–176.

    Google Scholar 

  210. Pfeil, W., 1977. Thermodynamical Approaches, to the Study of Proteins, lecture presented at the 5th International Conference on Chemical Thermodynamics, Work shop No. 8, The Conformational Thermodynamics of Biological Macromolecules, 23.− 26.8.1977, Ronneby, Sweden.

  211. Privalov, P. L. & Pfeil, W., 1979. Protein: Structure, Function and Industrial Applications, Proc. 12th FEBSMeeting Dresden (Hofmann, E., Pfeil, W. and Aurich, H., eds.), Pergamon Press, Oxford and New York, vol. 52, pp. 159–172.

    Google Scholar 

  212. Browne, W. J., North, A. C. T., Phillips, D. C., Vanaman, T. C. & Hill, R. L., 1969. J. Mol. Biol. 42, 65–86.

    Google Scholar 

  213. Warme, P. K., Momany, F. A., Rumball, S. V., Turtle, R. W. & Scheraga, H. A., 1974. Biochemistry 13, 768–782.

    Google Scholar 

  214. Iyer, K. S. & Klee, W. A., 1973. J. Biol. Chem. 248, 707–710.

    Google Scholar 

  215. Takesada, H., Nakanishi, M. & Tsuboi, M., 1973. J. Mol. Biol. 77, 605–614.

    Google Scholar 

  216. Ikegami, A., 1977, Biophys. Chem. 6, 117–130.

    Google Scholar 

  217. Kanehisa, M. I. & Ikegami, A., 1977. Biophys. Chem. 6, 131–149.

    Google Scholar 

  218. Cooper, A., 1976. Proc. Natl. Acad. Sci, USA 73, 2740–2741.

    Google Scholar 

  219. Privalov, P. L. & Tsalkova, T. N., 1979. Nature 280, 693–696.

    Google Scholar 

  220. Gurd, F. R. N. & Rothgeb, M., 1979. Adv. Protein Chem. 33, 73–165.

    Google Scholar 

  221. Northrupp, S. H., Pear, M. R., McCammon, J. A. & Karplus, M., 1980. Nature 286, 304–305.

    Google Scholar 

  222. Suezaki, Y. & Go, N., 1975. Int. J. Peptide Protein Res. 7, 333–334.

    Google Scholar 

  223. Ueda, Y. & Go, N., 1976. Int. J. Peptide Protein Res. 8, 551–558.

    Google Scholar 

  224. Sturtevant, J. M., 1977. Proc. Natl. Acad. Sci. USA 74, 2236–2240.

    Google Scholar 

  225. Franks, F., 1978. Characterisation of Protein Conformation and Function (Franks, F., ed.), Symposium Press, London, pp. 37–53.

    Google Scholar 

  226. Suurkuusk, J., 1974. Acta chem. Scand. Ser. B 28, 409–417.

    Google Scholar 

  227. Millero, F. J., Ward, G. K. & Chetirkin, P., 1976. J. Biol. Chem. 251, 4001–4004.

    Google Scholar 

  228. Millero, F. J., Ward, G. K. & Chetirkin, P., 1977. J. Biol. Chem. 252, 1127.

    Google Scholar 

  229. Yang, P.-H. & Rupley, J. A., 1979. Biochemistry 12, 2654–2661.

    Google Scholar 

  230. Scheraga, H. A., 1963. The Proteins (Neurath, H., ed.), Academic Press, New York and London, 2nd ed., Vol. I, pp. 477–594.

    Google Scholar 

  231. Baldwin, R. L. & Creighton, T. E., 1980. Protein Folding (Jaenicke, R., ed.), Elsevier/ North Holland Biomed. Press, Amsterdam and New York, pp. 217–260.

    Google Scholar 

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  1. Dept. of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences of the G.D.R., Lindenberger Weg 70, 1-1115, Berlin-Such, Germany

    Wolfgang Pfeil

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Pfeil, W. The problem of the stability of globular proteins. Mol Cell Biochem 40, 3–28 (1981). https://doi.org/10.1007/BF00230185

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